Synthesis and analysis of degradation, mechanical and toxicity properties of poly(β-amino ester) degradable hydrogels
Synthesis and analysis of degradation, mechanical and toxicity properties of poly(β-amino ester) degradable hydrogels
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DOI：
10.1016/j.actbio.2011.01.024
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发表时间：
2011-05-01
影响因子：
9.7
通讯作者：
Hilt, J. Zach
中科院分区：
文献类型：
--
作者：
Hawkins, Ashley M.;Milbrandt, Todd A.;Hilt, J. Zach
近年来，可生物降解水凝胶因其可调节的特性和与天然软组织的相似性，在生物医学应用（包括组织工程和药物递送）方面得到了广泛的研究。在这项工作中，合成了两种聚（B-氨基酯）可生物降解水凝胶系统并在体外进行了研究。观察到不同的降解曲线，从7小时到4个月不等，并且随着降解的进行，压缩模量显示出下降。μ细胞毒性分析用于分析细胞对降解产物的反应，水凝胶系统表现出与聚（dl-丙交酯-共-乙交酯）降解产物相似的毒性最后，通过将多能间充质细胞直接接种到水凝胶表面，然后进行活/死测定和荧光成像来研究细胞附着。细胞在24小时（98％）时显示出显着的活力，在48小时（72％）时稍低，但仍然具有很大的活力。这些水凝胶表现出一系列特性和有利的细胞反应，所有这些都表明这些材料可能是可行的用于组织再生和其他生物医学应用的材料 (C) 2011 Acta Materialia Inc 由 Elsevier Ltd 出版 保留所有权利
Biodegradable hydrogels have been studied extensively in recent years for biomedical applications, including tissue engineering and drug delivery, due to their tunable properties and similarities to natural soft tissue In this work, two poly(B-amino ester) biodegradable hydrogel systems were synthesised and studied in vitro Different degradation profiles were observed, ranging from 7 h to 4 months, and the compressive modulus was shown to decrease as degradation proceeded mu cytotoxicity analysis was used to analyze the cellular response to the degradation products, and the hydrogel systems exhibited similar toxicity to poly( dl-lactide-co-glycolide) degradation products Finally, cell attachment was studied by seeding pluripotent mesenchymal cells directly onto the hydrogel surfaces followed by a live/dead assay and fluorescent imaging Cells showed significant viability at 24 h (98%) and slightly lower, but still substantial viability, at 48 h (72%) These hydrogels exhibited a range of properties and favorable cellular responses, all which indicate that these materials could be viable materials for tissue regeneration and other biomedical applications (C) 2011 Acta Materialia Inc Published by Elsevier Ltd All rights reserved