INCORPORATION OF RADIOACTIVITY FROM (14C) GLUCOSE INTO SOLUBLE METABOLIC INTERMEDIATES OF MALARIA PARASITES
INCORPORATION OF RADIOACTIVITY FROM (14C) GLUCOSE INTO SOLUBLE METABOLIC INTERMEDIATES OF MALARIA PARASITES
复制标题
DOI：
10.4269/ajtmh.1964.13.515
复制
发表时间：
1964-01-01
影响因子：
3.3
通讯作者：
VOLLER, A
中科院分区：
文献类型：
--
作者：
BRYANT, C;SMITH, MJH;VOLLER, A
已经研究了[14C]葡萄糖和[1:4-14C2]琥珀酸的放射性掺入游离伯氏疟原虫的可溶性代谢中间体以及正常和寄生的小鼠血细胞中。游离寄生虫对标记葡萄糖的利用率较低，葡萄糖分解代谢的主要产物是乳酸。受感染的小鼠血细胞比正常细胞利用更多的葡萄糖，同位素的掺入模式表明寄生虫的存在增加了糖酵解和氧化反应。仅痕量的标记琥珀酸盐放射性被游离的伯氏疟原虫、正常小鼠血细胞和寄生细胞掺入可溶性中间体中。
The incorporation of radioactivity from [14C] glucose and [1:4-14C2] succinate into the soluble metabolic intermediates of free Plasmodium berghei and into normal and parasitized mouse blood cells has been studied. The free parasite exhibited a low utilization of the labeled glucose, and the main product of glucose catabolism was lactate. The infected mouse blood cell utilized considerably more glucose than did the normal cell, and the patterns of incorporation of the isotope suggests that the presence of the parasite increased both glycolytic and oxidative reactions. Only trace amounts of radioactivity from the labeled succinate were incorporated into soluble intermediates by free P. berghei, normal mouse blood cells, and parasitized cells.