Dietary soybean protein prevents bone loss in an ovariectomized rat model of osteoporosis
Dietary soybean protein prevents bone loss in an ovariectomized rat model of osteoporosis
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DOI：
10.1093/jn/126.1.161
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发表时间：
1996-01-01
影响因子：
4.2
通讯作者：
Kukreja, SC
中科院分区：
文献类型：
--
作者：
Arjmandi, BH;Alekel, L;Kukreja, SC
本研究的目的是检测大豆分离蛋白是否能预防卵巢激素缺乏引起的骨丢失。将32只95日龄Sprague-Dawley大鼠随机分为4个治疗组[假手术组（sham）;卵巢切除组（ovx）; ovx +大豆组; ovx + 17 β-雌二醇（E（2））组]，并在30天后处死。在假手术组、卵巢切除组和卵巢切除+ 17 β-雌二醇组中，大鼠喂食基于酪蛋白的饮食，大豆组喂食大豆分离蛋白而不是酪蛋白;饮食在其他方面相当。OVX组大鼠右侧股骨（P < 0.001）和第四腰椎（P < 0.05）的骨密度明显低于Sham组。在接受17 β-雌二醇或喂食大豆的动物中未观察到这些较低的骨密度。OVX组血清1，25-二羟胆钙化醇浓度也显著高于其他三组（P < 0.01）。我们的研究结果表明，膳食大豆蛋白是有效的预防骨质流失由于卵巢激素缺乏症。由于与假手术动物相比，ovx组和ovx +大豆组中碱性磷酸酶和抗酒石酸酸性磷酸酶的血清活性显着更高，但接受17 β-雌二醇的组中则不然，这证实了卵巢切除术增强和17 β-雌二醇抑制骨转换率。尽管大豆喂养动物的骨转换率较高，但这些大鼠的椎骨和股骨骨密度显著高于ovx组大鼠，表明骨形成超过骨吸收。需要进一步的研究来澄清这种对骨骼的保护作用是由于蛋白质本身还是由于大豆蛋白中的异黄酮的存在。
The purpose of this study was to examine whether soybean protein isolate prevents bone loss induced by ovarian hormone deficiency. Thirty-two 95-d-old Sprague-Dawley rats were randomly assigned to four treatment groups [sham-operated (sham); ovariectomized (ovx); ovx + soybean; ovx + 17 beta-estradiol (E(2))] and killed after 30 d. Rats in the sham, ovx and ovx + 17 beta-estradiol groups were fed a casein-based diet, and the soybean group was fed soybean protein isolate instead of casein; the diets were otherwise comparable. Rats in the ovx group had significantly lower densities of the right femur (P < 0.001) and the fourth lumbar vertebra (P < 0.05) than rats in the sham group. These lower bone densities were not observed in animals receiving 17 beta-estradiol or fed soybean. The ovx group also had significantly (P < 0.01) greater serum concentrations of 1,25-dihydroxycholecalciferol than the other three groups. Our findings suggest that dietary soybean protein is effective in preventing bone loss due to ovarian hormone deficiency. Because serum activities of both alkaline phosphatase and tartrate-resistant acid phosphatase were significantly greater in the ovx group and in the ovx + soybean group but not in the group receiving 17 beta-estradiol, compared with sham animals, this confirms that ovariectomy enhances and 17 beta-estradiol suppresses the rate of bone turnover. Despite the higher rate of bone turnover in the soybean-fed animals, the vertebral and femoral bone densities of these rats were significantly greater than those of rats in the ovx group, suggesting that formation exceeded resorption. Further studies are needed to clarify whether this protective effect on bone is due to the protein itself or to the presence of isoflavones in soybean protein.