Comparative BAC-based mapping in the white-throated sparrow, a novel behavioral genomics model, using interspecies overgo hybridization
Comparative BAC-based mapping in the white-throated sparrow, a novel behavioral genomics model, using interspecies overgo hybridization
复制标题
白喉麻雀基于 BAC 的比较作图，一种新型行为基因组学模型，使用种间杂交
作者：
Michael N Romanov;J. Dodgson;R. Gonser;E. Tuttle
背景基因组学时代已经产生了大量的测序生物资源，使研究人员能够瞄准那些没有可比性的映射和序列信息的物种。这些新的“非模式”生物体提供了独特的机会，研究环境对基因组模式和过程的影响。在这里，我们使用比较映射作为第一步，在一种新的动物模型，白喉麻雀（Zonotrichia albicollis），这是作为白色或棕褐色的变体，表现出替代的行为和生理特征的基因组组织的特征。形态是由复杂的染色体重排的存在与否决定的。该物种是行为基因组学的理想模型，因为基因型和表型之间的关联是绝对的，使得识别表型变异的基因组基础成为可能。发现我们通过与设计用于鸡、火鸡和斑胸草雀的overgo探针进行过滤杂交来表征白喉麻雀BAC库，从而启动了该物种的基因组研究。跨物种杂交导致640个阳性麻雀BAC被分配到77个鸡基因座，几乎所有的宏观和微观染色体，重点是与形态相关的染色体。出216 overgos，36%的探针杂交成功，平均数为3.0积极麻雀BAC每overgo.ConclusionsThese数据将被用于确定染色体结构和精细规模的映射与表型差异相关的候选基因。我们的研究证实了种间杂交在其他非模式生物中开发比较地图的实用性。
BackgroundThe genomics era has produced an arsenal of resources from sequenced organisms allowing researchers to target species that do not have comparable mapping and sequence information. These new "non-model" organisms offer unique opportunities to examine environmental effects on genomic patterns and processes. Here we use comparative mapping as a first step in characterizing the genome organization of a novel animal model, the white-throated sparrow (Zonotrichia albicollis), which occurs as white or tan morphs that exhibit alternative behaviors and physiology. Morph is determined by the presence or absence of a complex chromosomal rearrangement. This species is an ideal model for behavioral genomics because the association between genotype and phenotype is absolute, making it possible to identify the genomic bases of phenotypic variation.FindingsWe initiated a genomic study in this species by characterizing the white-throated sparrow BAC library via filter hybridization with overgo probes designed for the chicken, turkey, and zebra finch. Cross-species hybridization resulted in 640 positive sparrow BACs assigned to 77 chicken loci across almost all macro-and microchromosomes, with a focus on the chromosomes associated with morph. Out of 216 overgos, 36% of the probes hybridized successfully, with an average number of 3.0 positive sparrow BACs per overgo.ConclusionsThese data will be utilized for determining chromosomal architecture and for fine-scale mapping of candidate genes associated with phenotypic differences. Our research confirms the utility of interspecies hybridization for developing comparative maps in other non-model organisms.
影响因子：
3.1
作者：
TIERSCH, TR;WACHTEL, SS
通讯作者：
WACHTEL, SS
影响因子：
56.9
作者：
Crabbe, JC;Wahlsten, D;Dudek, BC
通讯作者：
Dudek, BC