Characterization of agronomy, grain physicochemical quality, and nutritional property of high-lysine 35R transgenic rice with simultaneous modification of lysine biosynthesis and catabolism
Characterization of agronomy, grain physicochemical quality, and nutritional property of high-lysine 35R transgenic rice with simultaneous modification of lysine biosynthesis and catabolism
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同时修饰赖氨酸生物合成和分解代谢的高赖氨酸35R转基因水稻农学、籽粒理化品质和营养特性的表征
DOI：
10.1021/acs.jafc.7b00621
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发表时间：
2017
影响因子：
6.1
通讯作者：
Liu Qiao-Quan
中科院分区：
文献类型：
--
作者：
Yang Qing-Qing;Wu Hong-Yu;Li Qian-Feng;Duan Ru-Xu;Zhang Chang-Quan;Sun Samuel Sai-Ming;Liu Qiao-Quan
赖氨酸是大米中第一种限制性必需氨基酸。我们以前构建了一系列转基因水稻品系，以增强赖氨酸的生物合成(35S)，下调其分解代谢(Ri)，或同时实现这两种代谢效应(35R)。在这项研究中，选择了9个转基因品系，每组3个，用于田间和动物饲养试验。结果表明，转基因S对水稻的田间表现和主要农艺性状无明显影响。转基因品系35R-17成熟种子的游离赖氨酸含量是野生型的48~60倍，表观直链淀粉含量略低，胶稠度较软。此外，35天的饲养实验表明，与野生型大米饲料相比，35R-17转基因大米饲料大鼠的体重增加、食物效率和蛋白质效率比都有所提高。这些数据将为35R高赖氨酸转基因水稻的进一步评价和商业化奠定基础。
Lysine is the first limiting essential amino acid in rice. We previously constructed a series of transgenic rice lines to enhance lysine biosynthesis (35S), down-regulate its catabolism (Ri), or simultaneously achieve both metabolic effects (35R). In this study, nine transgenic lines, three from each group, were selected for both field and animal feeding trials. The results showed that the transgene(s) caused no obvious effects on field performance and main agronomic traits. Mature seeds of transgenic line 35R-17 contained 48–60-fold more free lysine than in wild type and had slightly lower apparent amylose content and softer gel consistency. Moreover, a 35-day feeding experiment showed that the body weight gain, food efficiency, and protein efficiency ratio of rats fed the 35R-17 transgenic rice diet were improved when compared with those fed wild-type rice diet. These data will be useful for further evaluation and potential commercialization of 35R high-lysine transgenic rice.