Patterning the embryonic heart:: Identification of five mouse Iroquois homeobox genes in the developing heart
Patterning the embryonic heart:: Identification of five mouse Iroquois homeobox genes in the developing heart
复制标题
DOI：
10.1006/dbio.2000.9801
复制
发表时间：
2000-08-15
影响因子：
2.7
通讯作者：
Moorman, AFM
中科院分区：
文献类型：
--
作者：
Christoffels, VM;Keijser, AGM;Moorman, AFM
我们分离了小鼠易洛魁族相关的同源异型盒基因Irx 1，-2-3，-4和-5的cDNA，并表征了它们在发育中的心脏中的表达模式。Irx 1和Irx 2被发现是专门表达在室间隔从其形成的开始向前。在胎儿阶段，这两种基因的表达似乎逐渐局限于房室束的心肌和形成心室传导系统的束支。Irx 3被发现在心室的小梁化心肌中特异性表达。Irx 4在线形心管和房室管以及心室肌的一段中观察到表达，包括成环后的内弯曲，类似于MLC 2 V的模式。Irx 5的转录物在心室和心房工作心肌的内膜中特异性检测到，该内膜也表达冯维勒布兰德因子，但在内膜垫的内膜中不存在，即，房室管、内弯和流出道。Irx 5的空间发育模式与心钠素的空间发育模式相匹配，心钠素是心室形成工作心肌的标志物。总之，发现Irx基因家族的所有成员在发育中的小鼠心脏中以高度特异性的模式表达，这表明在四室心脏的不同组成部分的规范中起着关键作用。(C)北京大学出版社.
We isolated cDNAs of mouse Iroquois-related homeobox genes Irx1, -2 -3, -4, and -5 and characterized their patterns of expression in the developing heart. Irx1 and Irx2 were found to be expressed specifically in the ventricular septum from the onset of its formation onward. In fetal stages, the expression of both genes appeared to gradually become confined to the myocardium of the atrioventricular bundle and bundle branches of the forming ventricular conduction system. Irx3 was found to be expressed specifically in the trabeculated myocardium of the ventricles. Irx4 expression was observed in a segment of the linear heart tube and the atrioventricular canal and ventricular myocardium including the inner curvature after looping, resembling the pattern of MLC2V. Transcripts for Irx5 were detected specifically in the endocardium lining the ventricular and atrial working myocardium that also expressed von Willebrand factor, but were absent from the endocardium of the endocardial cushions, i.e., the atrioventricular canal, inner curvature, and outflow tract. The spatiodevelopmental pattern of Irx5 matched that of ANF, a marker for the forming working myocardium of the chambers. Taken together, all members of the Irx gene family were found to be expressed in highly specific patterns in the developing mouse heart, suggesting a critical role in the specification of the distinct components of the four-chambered heart. (C) 2000 Academic Press.