VILLOUS ATROPHY, CRYPT HYPERPLASIA, CELLULAR INFILTRATION, AND IMPAIRED GLUCOSE-NA ABSORPTION IN ENTERIC CRYPTOSPORIDIOSIS OF PIGS
VILLOUS ATROPHY, CRYPT HYPERPLASIA, CELLULAR INFILTRATION, AND IMPAIRED GLUCOSE-NA ABSORPTION IN ENTERIC CRYPTOSPORIDIOSIS OF PIGS
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DOI：
10.1016/0016-5085(90)90325-u
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发表时间：
1990-05-01
期刊：
影响因子：
29.4
通讯作者：
POWELL, DW
中科院分区：
文献类型：
--
作者：
ARGENZIO, RA;LIACOS, JA;POWELL, DW
在隐孢子虫病的新生猪模型中检查了肠道形态以及液体和电解质的转运。在感染后第3、6、9和12天获得空肠、回肠和结肠切片用于形态学分析，并在感染后第3和4天进行空肠和回肠的体内灌注研究。第3天，最严重的形态学病变出现在回肠，包括绒毛萎缩、隐窝增生和细胞浸润。绒毛表面积从2.1±减少。 0.4 倍105 .mu.m2 控制回肠至 0.8 .+-。 0.1.次。受感染的回肠中为105μm2，该结果与肠上皮细胞数量较少且横截面积减小有关。相反，绒毛固有层中的炎性细胞数量从456个+-增加。 116控制到1014.+-。 187 在受感染的绒毛中，固有层体积没有显着变化。在感染高峰期，微生物和炎症细胞与绒毛肠细胞的比例约为 1:2。相反，在隐窝中没有观察到生物体，并且隐窝固有层中炎症细胞的浓度没有改变。回肠中生物体和多形核细胞的消失与正常结构的恢复有关，并在第 12 天完成。虽然在结肠中看到了生物体，但总体结构并未受到严重影响。感染后第 3 天和第 4 天，受感染猪的空肠和回肠中葡萄糖刺激的钠和水的吸收完全受损；然而，在没有葡萄糖的情况下，从碱性林格氏溶液中吸收电解质和水并没有受到显着影响。这些结果与与形态损伤相关的吸收不良性腹泻病一致，并且与猪肠道病毒性疾病中观察到的结果非常相似，只是后者的上肠受到更严重的影响。
Intestinal morphology and fluid and electrolyte transport were examined in a neonatal porcine model of cryptosporidiosis. Sections of jejunum, ileum, and colon were obtained for morphometric analysis on days 3, 6, 9, and 12 postinfection, and in vivo perfusion studies of jejunum and ileum were conducted on days 3 and 4 postinfection. The most severe morphologic lesion was seen in the ileum on day 3, and consisted of villous atrophy, crypt hyperplasia, and cellular infiltration. Villous surface area was reduced from 2.1 .+-. 0.4 .times. 105 .mu.m2 in control ileum to 0.8 .+-. 0.1 .times. 105 .mu.m2 in infected ileum, a result associated with enterocytes that were fewer in number and reduced in cross-sectional area. Conversely, the number of inflammatory cells in the lamina propria of the villus increased from 456 .+-. 116 in control to 1014 .+-. 187 in infected villus without a significant change in the volume of the lamina propria. At the height of infection, there was an approximate 1:2 ratio of both organisms and inflammatory cells to villous enterocytes. In contrast, organisms were not observed in the crypts, and the concentration of inflammatory cells in crypt lamina propria was unaltered. Disappearance of organisms and polymorphonuclear cells from the ileum was associated with restoration of normal structure and was complete by day 12. Although organisms were seen in the colon, the general architecture was not severely affected. On days 3 and 4 postinfection, there was a complete impairment of the glucose-stimulated Na and water absorption in both jejunum and ileum of infected pigs; however, absorption of electrolytes and water from a basic Ringer''s solution, in the absence of glucose, was not significantly affected. These results are consistent with a malabsorptive diarrheal disease associated with the morphological damage and are very similar to those seen in enteric viral disease in pigs, except that the upper intestine is more severely affected in the latter.