Gsdma3 gene is needed for the induction of apoptosis-driven catagen during mouse hair follicle cycle
Gsdma3 gene is needed for the induction of apoptosis-driven catagen during mouse hair follicle cycle
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Gsdma3 基因是诱导小鼠毛囊周期中细胞凋亡驱动的退行期所必需的
DOI：
10.1007/s00418-011-0845-8
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发表时间：
2011-09-01
影响因子：
2.3
通讯作者：
Lian, Xiaohua
中科院分区：
文献类型：
--
作者：
Lei, Mingxing;Gao, Xiang;Lian, Xiaohua
Gsdm是一个新发现的基因家族，其表达局限于胃肠道和皮肤上皮。Gsdma 3是Gsdma亚家族的主要成员之一，在小鼠皮肤毛囊中特异性表达，但其功能尚不清楚。苏木精-伊红染色结果显示，Gsdma 3基因突变可导致毛发生长进入退行期，毛囊长度不缩短，结构不收缩，退行期发育受到抑制。TUNEL染色进一步显示突变小鼠毛囊的凋亡明显减少。免疫荧光、Western blot和RT-PCR检测突变小鼠毛囊中Caspase-3表达下调。经皮内注射Gsdma 3基因表达质粒后，突变小鼠毛囊中的细胞凋亡和Caspase-3的表达增强，从而挽救了Gsdma 3突变小鼠的退行期延迟。我们的研究结果证实，Gsdma 3基因突变在小鼠毛囊周期中干扰退行期的形成，通过上调Caspase-3的表达和促进细胞凋亡，Gsdma 3基因可以在正常的退行期诱导中发挥重要作用。
Gsdm is a newly found gene family, which is restricted in its expression to the gastrointestinal tract and the skin epithelium. As a main member of the Gsdma subfamily, Gsdma3 is expressed specifically in the hair follicle of mouse skin, but its function remains largely unclear. By hematoxylin and eosin staining, we showed that Gsdma3 gene mutation caused an abnormal catagen phase with unshortened length and unshrunk structure of the hair follicle, in which the development of catagen phase was inhibited. TUNEL staining further revealed that the apoptosis of the hair follicle was obviously decreased in mutant mice. Caspase-3 downregulation was also detected by immunofluorescence, Western blot and RT-PCR in the hair follicle of the mutant mice. After intradermal injection of Gsdma3 gene expression plasmid, apoptosis as well as Caspase-3 expression in the hair follicle of mutant mice was enhanced, and so the catagen retardation of Gsdma3-mutant mice was rescued. Our results confirmed that Gsdma3 gene mutation interfered with catagen formation during mouse hair follicle cycle and, by upregulation of Caspase-3 expression and promotion of apoptosis, Gsdma3 gene could play an essential role in normal catagen induction.