Autophagy is required for CSF-1-induced macrophagic differentiation and acquisition of phagocytic functions
Autophagy is required for CSF-1-induced macrophagic differentiation and acquisition of phagocytic functions
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DOI：
10.1182/blood-2011-11-392167
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发表时间：
2012-05-10
期刊：
影响因子：
20.3
通讯作者：
Auberger, Patrick
中科院分区：
文献类型：
--
作者：
Jacquel, Arnaud;Obba, Sandrine;Auberger, Patrick
自噬是溶酶体降解多余或受损的大分子或细胞器的过程。药理学和遗传学证据表明，自噬在细胞的稳态、发育、存活和分化中起着多效性作用。人血单核细胞向巨噬细胞的分化是一个半胱天冬酶依赖的过程时，触发离体集落刺激因子-1。我们在这里显示，使用药理学抑制剂，siRNA方法，和Atg 7（-/-）小鼠，自噬启动ULK 1是需要适当的集落刺激因子-1驱动的分化的人和鼠单核细胞。我们还阐明了自噬在巨噬细胞获得吞噬功能中的作用。总的来说，这些发现强调了自噬在单核细胞分化和巨噬细胞功能获得过程中意想不到的重要作用。（血。2012; 119（19）：4527-4531）
Autophagy is the process by which superfluous or damaged macromolecules or organelles are degraded by the lysosome. Pharmacologic and genetic evidence indicates that autophagy plays pleiotropic functions in cellular homeostasis, development, survival, and differentiation. The differentiation of human blood monocytes into macrophages is a caspase-dependent process when triggered ex vivo by colony stimulating factor-1. We show here, using pharmacologic inhibitors, siRNA approaches, and Atg7(-/-) mice, that autophagy initiated by ULK1 is required for proper colony stimulating factor-1-driven differentiation of human and murine monocytes. We also unravel a role for autophagy in macrophage acquisition of phagocytic functions. Collectively, these findings highlight an unexpected and essential role of autophagy during monocyte differentiation and acquisition of macrophage functions. (Blood. 2012; 119( 19): 4527-4531)