IMMUNOLOGICAL ANALYSIS OF RABBIT ANTI-ANTIBODY SYSTEMS
IMMUNOLOGICAL ANALYSIS OF RABBIT ANTI-ANTIBODY SYSTEMS
复制标题
兔抗抗体系统的免疫学分析
DOI：
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发表时间：
1968
影响因子：
15.3
通讯作者：
P. G. H. Cell
中科院分区：
文献类型：
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作者：
A. Kelus;P. G. H. Cell
用19个兔抗寻常变形杆菌x 19抗体样品吸附在杆菌上，对39只兔免疫过程中产生的抗独特型抗体（抗抗体）进行了研究。除了常规的抗独特型抗体外，还经常出现针对IgG以外的免疫球蛋白类分子的抗同种异体抗体，特别是在这种免疫过程中可能出现的巨球蛋白。在四个病例中，正如预期的那样，试图提高自身抗独特型抗体是不成功的。独特型特异性（抗原决定因子）主要在IgG上发现，但有时也在IgM分子上发现。抗独特型抗体的个体特异性似乎是绝对的，只要识别和排除抗同种异体抗体；例如，每一种独特型特异性仅为一只兔子和该兔子体内的一种抗体所特有。这些原则甚至在兔家族的抗体被检查时也成立：亲代独特型决定因素在后代中找不到。在两个样本中，IgG分子的Fab片段存在独特型特异性，而抗体结合位点不存在独特型特异性。
A study has been made of the production of antiidiotypic antibodies (anti-antibodies) arising during the immunization of 39 rabbits with 19 individual samples of rabbit anti-Proteus vulgaris x 19 antibodies adsorbed onto bacilli. In addition to the regular demonstration of antiidiotypic antibodies attention is drawn to the frequent occurrence of antiallotypic antibodies against molecules of immunoglobulin classes other than IgG, especially macroglobulins, which may arise during such immunization. In four cases attempts to raise autoantiidiotypic antibodies were unsuccessful, as expected. The idiotypic specificities (antigenic determinants) have been found mainly on IgG but also sometimes on IgM molecules. The individual specificity of the antiidiotypic antibodies appears to be absolute, as long as the antiallotypic antibodies are recognized and excluded; e.g., each idiotypic specificity is characteristic of only one single rabbit and of a single kind of antibody within that rabbit. These principles hold even when antibodies of rabbit families are examined: the parental idiotypic determinants could not be found in the offspring. In two samples tested the idiotypic specificities were found on the Fab fragment of IgG molecule but not on its antibody-combining site.