The effect of inherited hydrocephalus and shunt treatment on cortical pyramidal cell dendrites in the infant H-Tx rat
The effect of inherited hydrocephalus and shunt treatment on cortical pyramidal cell dendrites in the infant H-Tx rat
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DOI：
10.1006/exnr.1996.0161
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发表时间：
1996-10-01
影响因子：
5.3
通讯作者：
Jones, HC
中科院分区：
文献类型：
--
作者：
Harris, NG;McAllister, JP;Jones, HC
婴儿脑积水的神经功能缺损的神经基础知之甚少。在听觉皮层锥体细胞的树突状结构的变化已被测量在出生后21天在H-Tx大鼠。通过快速凝胶法从脑积水和对照同窝仔以及出生后3-4天或10天放置脑室皮下分流的脑积水大鼠制备组织。在光学显微镜下以250或400倍的放大率定量分析第V层锥体细胞。与对照组相比，脑积水大鼠的皮质厚度减少了30%，而分流治疗组大鼠的皮质厚度与对照组相似。对于顶树突和基底树突，脑积水大鼠从索马延伸的距离减少了49- 57%，树突树的总长度分别减少了61%和77%。在3-4天进行分流处理的大鼠有小的树突变化，在大多数情况下，与对照组没有显著差异。在10天时接受分流治疗的大鼠具有与未治疗的脑积水大鼠无法区分的树突。树突状分支模式也受到影响;分支节段的数量和平均长度在脑积水组和10天分流组中均减少，仅在早期组中有微小变化。总体而言，基底树突比顶端树突受到更严重的影响。它的结论是，婴儿脑积水导致严重的神经元异常，可以在很大程度上，但不完全，在早期阶段进行分流治疗，以防止。(C)出版社：Academic Press，Inc.
The neuronal basis for neurological deficits in infantile hydrocephalus is poorly understood. Changes in the dendritic architecture of pyramidal cells of the auditory cortex have been measured at 21 days after birth in H-Tx rats. Tissue was prepared by the rapid Gels method from hydrocephalic and control littermates, together with hydrocephalic rats with ventriculo-subcutaneous shunts placed at 3-4 days or at 10 days after birth. Layer V pyramidal cells were analyzed quantitatively on a light microscope at a magnification of 250 or 400 x. When compared to control, the hydrocephalic rats had a 30% reduction in the cortical thickness whereas in the shunt-treated rats it was similar to control. For both the apical and the basal dendrites, the distance extended from the soma was reduced in hydrocephalic rats by 49-57%, and the total length of the dendritic trees was decreased by 61 and 77%, respectively. Rats shunt-treated at 3-4 days had small dendrite changes which, in most cases, were not significantly different from control. Rats shunt-treated at 10 days had dendrites which were indistinguishable from untreated hydrocephalic rats. Dendritic branch patterns were also affected; the number and mean length of branch segments were reduced in both the hydrocephalic and the 10-day shunt group, with only small changes in the earlier group. Overall, the basal dendrites were more severely affected than the apical dendrites. It is concluded that infantile hydrocephalus results in severe neuronal abnormalities which can largely, but not completely, be prevented by shunt treatment performed in the early stages. (C) 1996 Academic Press, Inc.