Retinoic acid-inducible gene-I is induced by interleukin-1β in cultured human gingival fibroblasts
Retinoic acid-inducible gene-I is induced by interleukin-1β in cultured human gingival fibroblasts
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DOI：
10.1111/j.1399-302x.2005.00181.x
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发表时间：
2005-02-01
影响因子：
--
通讯作者：
Kimura, H
中科院分区：
文献类型：
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作者：
Sakaki, H;Imaizumi, T;Kimura, H
视黄酸诱导基因-I(RIG-I)是DExH盒家族蛋白的一员，其生物学功能尚不清楚。我们研究了IL-1β(IL-1β)诱导人牙龈成纤维细胞RIG-I表达的机制。我们还探讨了在过表达RIG-I的成纤维细胞中COX-2、RANTES和Galectin-9表达增强的可能性。用IL-1β刺激培养的人牙龈成纤维细胞，用逆转录聚合酶链式反应和Western印迹分析检测RIG-I的mRNA和蛋白的表达。观察蛋白质合成抑制剂放线菌酮对IL-1β诱导的RIG-I表达的影响。同时检测转染RIG-I基因的牙周成纤维细胞中COX-2、RANTES、Galectin-9和单核细胞趋化蛋白-1的表达。IL-1β以时间和浓度依赖的方式刺激培养的成纤维细胞RIG-I的mRNA和蛋白表达。放线菌酮不能抑制IL-1β诱导的RIG-I的表达。RIG-I基因导入成纤维细胞后，COX-2mRNA的表达增强，RANTES和Galectin-9mRNA的表达略有增加。相反，RIG-I的过度表达并没有改变单核细胞趋化蛋白-1的mRNA水平。我们得出结论：IL-1β刺激人牙龈成纤维细胞RIG-I的表达。
Retinoic acid-inducible gene-I (RIG-I) is a member of the DExH box family protein, and details of its biological function are not known. We have studied the mechanism of the interleukin-1beta (IL-1beta)-induced RIG-I expression in human gingival fibroblasts in culture. We also addressed the possibility of enhanced expression of COX-2, RANTES and galectin-9 in fibroblasts overexpressed RIG-I. We stimulated cultured human gingival fibroblasts with IL-1beta and examined the expression of RIG-I mRNA and protein by reverse transcriptase-mediated polymerase chain reaction and Western blot analysis. The effect of cycloheximide, a protein synthesis inhibitor, on the IL-1beta-induced expression of RIG-I was examined. The expression of COX-2, RANTES, galectin-9 and monocyte chemoattractant protein-1 in gingival fibroblasts transfected with RIG-I cDNA was also examined. IL-1beta stimulated the expressions of mRNA and protein for RIG-I, in cultured fibroblasts, in a time- and concentration-dependent manner. Cycloheximide did not suppress the IL-1beta-induced RIG-I expression. Introduction of RIG-I cDNA into fibroblasts resulted in enhanced expression of COX-2 mRNA, and slightly enhanced the expression of mRNA for RANTES and galectin-9. In contrast, RIG-I overexpression did not alter the level of mRNA for monocyte chemoattractant protein-1. We conclude that IL-1beta stimulates RIG-I expression in human gingival fibroblasts.