Flow cytometric analysis of intracellular chemokines in chondrocytes in vivo:: constitutive expression and enhancement in osteoarthritis and rheumatoid arthritis
Flow cytometric analysis of intracellular chemokines in chondrocytes in vivo:: constitutive expression and enhancement in osteoarthritis and rheumatoid arthritis
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DOI：
10.1016/s0014-5793(99)00886-8
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发表时间：
1999-07-23
期刊：
影响因子：
3.5
通讯作者：
Facchini, A
中科院分区：
文献类型：
--
作者：
Borzì, RM;Mazzetti, I;Facchini, A
趋化因子在调节炎症部位的白细胞功能中起着关键作用。为探讨软骨细胞在炎症关节趋化环境中的作用，采用流式细胞仪和RT-PCR方法检测了软骨细胞中CC和CXC趋化因子的含量，并用流式细胞仪和RT-PCR方法检测了软骨细胞中IL-8、Gro-α、MCP-1、RANTES、MIP-1α和MIP-1β的表达。除RANTES外，其余趋化因子在正常软骨细胞中均有不同程度的表达。在骨关节炎和类风湿关节炎患者中，IL-8、Groα、MIP-1α和MIP-1β均有不同程度的增强。(C)1999年欧洲生化学会联合会。
Chemokines play a key role in modulating leukocyte functions at sites of inflammation. To assess chondrocyte contribution to the chemotactic environment of inflamed joints the intracellular content of CC and CXC chemokines mas investigated, IL-8, GRO alpha MCP-1, RANTES, MIP-1 alpha and MIP-1 beta expression was evaluated by flow cytometric analysis and RT-PCR in chondrocytes isolated from cartilage specimens obtained from patients with osteoarthritis and rheumatoid arthritis and multiorgan donors as normal controls. All the chemokines except RANTES were found in normal chondrocytes, with different degrees of staining intensity, In osteoarthritis and rheumatoid arthritis patients, an enhancement of IL-8, GRO alpha, MIP-1 alpha and MIP-1 beta was observed. (C) 1999 Federation of European Biochemical Societies.