Cell transplantation to prevent heart failure: A comparison of cell types
Cell transplantation to prevent heart failure: A comparison of cell types
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DOI：
10.1016/s0003-4975(03)01013-0
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发表时间：
2003-12-01
影响因子：
4.6
通讯作者：
Li, RK
中科院分区：
文献类型：
--
作者：
Fujii, T;Yau, TM;Li, RK
背景。心肌梗死后自体细胞移植可恢复可存活的肌肉。我们比较了三种细胞类型或一种血管紧张素转换酶（ACE）抑制剂对心脏损伤后心室功能的保护作用。冻伤在成年大鼠身上形成均匀的跨壁心肌瘢痕。三周后，大鼠被随机分配到四种盲法治疗中的一种：移植5 × 106个主动脉平滑肌细胞（SMC, n = 12）、心室心脏细胞（VHC, n = 13）、骨骼肌细胞（SKC, n = 13）或单独培养基（对照组，n = 11）。ACE抑制剂组（n = 8）同样在冷冻损伤后3周开始使用依那普利（1.0 mg/kg /天）。移植后5周和12周，在Langendorff仪器中评估左心室（LV）功能，并对左心室疤痕进行组织学和免疫组织学评估。5周时，细胞移植或ACE抑制剂治疗组瘢痕弹性蛋白含量较高，左室功能较对照组改善（p < 0.05）。移植后12周，细胞移植大鼠的弹性蛋白含量和左室功能仍高于对照组大鼠，但ACE抑制剂治疗大鼠的弹性蛋白含量和左室功能均低于对照组（p < 0.05）。SMC、VHC和SKC移植保留的心室功能相当于ACE抑制剂的作用。肌肉细胞移植，而不是ACE抑制剂治疗，在冷冻损伤后继续有效。肌肉细胞之间没有发现差异。(C) 2003年由胸外科学会出版。
Background. Autologous cell transplantation may restore viable muscle after a myocardial infarction. We compared the effect of three cell types or an angiotensin-converting enzyme (ACE) inhibitor on preservation of ventricular function after cardiac injury.Methods. A uniform transmural myocardial scar was created in adult rats by cryoinjury. Three weeks later the rats were randomly assigned to one of four blinded treatments: transplantation with 5 X 106 aortic smooth muscle cells (SMC, n = 12), ventricular heart cells (VHC, n = 13), skeletal muscle cells (SKC, n = 13) or culture medium alone (control, n = 11). The ACE inhibitor group (n = 8) received enalapril (1.0 mg/kg per day), also beginning 3 weeks after cryoinjury. Five and 12 weeks after transplantation, left ventricle (LV) function was assessed in a Langendorff apparatus, and histologic and immunohistological evaluation of the LV scars was performed.Results. At 5 weeks, greater scar elastin content and better LV function was noted with cell transplantation or ACE inhibitor therapy compared with control rats (p < 0.05). Twelve weeks after transplantation, cell-transplanted rats still had greater elastin content and better LV function than control rats, although elastin content and LV function had declined in ACE inhibitor-treated animals to levels below those observed in control rats (p < 0.05).Conclusions. Transplantation of SMC, VHC, and SKC preserved ventricular function equivalent to the effects of an ACE inhibitor. Muscle cell transplantation, but not ACE inhibitor therapy, continues to be effective later after cryoinjury. No differences were detected between the muscle cells. (C) 2003 by The Society of Thoracic Surgeons.