Polysaccharide from Gracilaria Lemaneiformis prevents colitis in Balb/c mice via enhancing intestinal barrier function and attenuating intestinal inflammation
Polysaccharide from Gracilaria Lemaneiformis prevents colitis in Balb/c mice via enhancing intestinal barrier function and attenuating intestinal inflammation
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DOI：
10.1016/j.foodhyd.2020.106048
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发表时间：
2020-12-01
影响因子：
10.7
通讯作者：
Lin, Zhiqi
中科院分区：
文献类型：
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作者：
Han, Rui;Wang, Li;Lin, Zhiqi
探讨龙须菜多糖对葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎的预防作用。红外光谱分析表明，SP是一种典型的硫酸化多糖（SP），能谱（EDS）分析表明SP中含有较高的S元素。SP主要由（1 -> 3）糖苷键组成。结果表明，SP可通过减轻体重下降和食欲抑制来改善小鼠的体质状况。SP还能降低血清内毒素（ET）、脂多糖结合蛋白（LBP）含量及髓过氧化物酶活性。同时，SP还抑制结肠组织中肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-1 β（IL-1 β）的分泌。这些指标表明SP改善了结肠炎症状。此外，组织学分析表明，SP可以维持健康的结肠显微结构，包括隐窝，杯状细胞和粘膜肌层。免疫组化结果显示，SP处理后结肠紧密连接蛋白Claudin-1、ZO-1和粘蛋白MUC-2表达增强。结论：SP具有增强肠屏障功能、保护结肠微结构、减轻DSS诱导的结肠损伤的作用，提示SP可能是一种潜在的抗结肠炎活性成分。
This study explored the preventive effects of polysaccharide extracted from Gracilaria Lemaneiformis in mice with colitis induced by dextran sulfate sodium (DSS). FT-IR spectrum showed that SP was a typical sulfated polysaccharide (SP), which contained high content of S element in energy dispersive spectrometer (EDS) analysis. SP was mainly composed of (1 -> 3)-linked glycosidic bonds. Our results showed that SP could improve physical conditions of mice via alleviating weight loss and appetite suppression. Besides, SP could decrease the content of endotoxin (ET), lipopolysaccharide binding protein (LBP) and the activity of myeloperoxidase in serum. Meanwhile, SP also suppressed the secretion of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and interleukin-1 beta (IL-1 beta) in colon tissue. These indicators showed that SP had ameliorated colitis symptoms. Furthermore, histological analysis indicated that SP could maintain healthy colonic microstructure including crypt, goblet cell and mucosal muscularis. Meanwhile, immunohistochemical results showed that tight junction proteins, including Claudin-1, ZO-1, and mucin (MUC-2) were promoted in colon with SP treatment. In conclusion, SP could enhance intestinal barrier, protect colonic microstructure and attenuate DSS-induced colonic injuries in mice, which suggested that SP could be a potentially functional component against colitis.