Derivation and morphological characterization of mouse spermatogonial stem cell lines
Derivation and morphological characterization of mouse spermatogonial stem cell lines
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DOI：
10.1679/aohc.67.297
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发表时间：
2004-11-01
影响因子：
--
通讯作者：
Kubota, Y
中科院分区：
文献类型：
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作者：
Ogawa, T;Ohmura, M;Kubota, Y
精原干细胞（SSC）尚未具备决定性标记，只能通过移植试验进行回顾性检测。最近有报道称，从 DBA/2 和 ICR 新生儿中收集的小鼠生殖母细胞可在体外繁殖。这种培养的生殖细胞被称为生殖系干细胞（GS细胞），当移植到受体小鼠睾丸中时，它会产生功能性精子以产生后代。在这里，我们证明 GS 细胞系不仅可以在新生小鼠的前睾丸中建立，而且可以从成年小鼠的睾丸中建立。我们还证实，GS细胞一旦移植到宿主睾丸中就可以恢复并恢复体外扩增，这表明它们可以与成人睾丸中的SSC相互转化。共聚焦激光显微镜检查显示 GS 细胞类似于成人睾丸中未分化的精原细胞。这种独特的细胞系可用于生殖细胞生物学研究，并可用作动物基因工程的新工具。
Spermatogonial stem cells (SSCs), having yet to possess decisive markers, can only be detected retrospectively by transplantation assay. It was reported recently that mouse gonocytes collected from DBA/2 and ICR neonates propagated in vitro. This cultured germ cell, named the germline stem cell (GS cell), produced functional sperm to make progeny when transplanted into recipient mouse testes. Here we show that GS cell lines can be established not only front neonatal testes but also from the testis of adult mice. We also confirmed that GS cells once transplanted into a host testis can be recovered to resume in vitro expansion, indicating that they are convertible mutually with SSCs in adult testes. Confocal laser microscopic examination showed GS cells resemble undifferentiated spermatogonia in the adult testis. This unique cell line could be useful for research in germ cell biology and applicable as a new tool for the genetic engineering of animals.