Discovery of a P450-catalyzed step in vindoline biosynthesis: a link between the aspidosperma and eburnamine alkaloids
Discovery of a P450-catalyzed step in vindoline biosynthesis: a link between the aspidosperma and eburnamine alkaloids
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DOI：
10.1039/c5cc01309g
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发表时间：
2015-01-01
影响因子：
4.9
通讯作者：
O'Connor, Sarah E.
中科院分区：
文献类型：
--
作者：
Kellner, Franziska;Geu-Flores, Fernando;O'Connor, Sarah E.
在这里，我们报告的细胞色素P450的发现，这是必要的生物合成的文多灵，一种植物来源的天然产物，用于半合成的几种抗癌药物。这种酶催化环氧化物的形成，该环氧化物可以进行重排以产生长春胺-eburnamine骨架，从而为长期存在的假设提供证据，即aspidosperma和eburnamine型生物碱是生物合成相关的。
Here we report the discovery of a cytochrome P450 that is required for the biosynthesis of vindoline, a plant-derived natural product used for semi-synthesis of several anti-cancer drugs. This enzyme catalyzes the formation of an epoxide that can undergo rearrangement to yield the vincamine-eburnamine backbone, thereby providing evidence for the long-standing hypothesis that the aspidosperma- and eburnamine-type alkaloids are biosynthetically related.