Kinetoplastid RNA editing ligases: complex association, characterization, and substrate requirements
Kinetoplastid RNA editing ligases: complex association, characterization, and substrate requirements
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DOI：
10.1016/s0166-6851(02)00333-x
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发表时间：
2003-04-03
影响因子：
1.5
通讯作者：
Stuart, K
中科院分区：
文献类型：
--
作者：
Palazzo, SS;Panigrahi, AK;Stuart, K
RNA编辑通过插入和删除尿苷酸（Us）来产生功能性mrna，对着丝粒线粒体转录物进行转录后处理。对催化编辑的多酶复合物（编辑体）中的RNA连接酶的活性和重组蛋白的活性进行了表征，发现它们是相似的。当用互补的RNA或DNA桥接时，两个RNA片段的连接增强，没有留下空隙或悬垂。在没有外源ATP的情况下，观察到G b> U > C > A的受体核苷酸偏好，但在添加ATP后，U优先，连接酶活性增加。底物特异性和催化特性表明，RNA连接酶的活性有助于RNA编辑的准确性。(C) 2003 Elsevier Science B.V.版权所有
RNA editing processes kinetoplastid mitochondrial transcripts post-transcriptionally by inserting and deleting uridylates (Us) to produce functional mRNAs. The activities of the RNA ligases in the multienzyme complex (the editosome) that catalyzes editing and of the recombinant proteins were characterized and found to be similar. Ligation of two RNA fragments was enhanced when bridged by a complementary RNA or DNA, which left no gaps or overhangs. An acceptor nucleotide preference of G > U > C > A was observed in the absence of exogenous ATP but U was preferred upon addition of ATP and ligase activity was increased. The substrate specificity and catalytic characteristics indicate that RNA ligase activity contributes to the accuracy of RNA editing. (C) 2003 Elsevier Science B.V. All rights reserved.