Developing VUV spectroscopy for protein folding and material luminescence on beamline 4B8 at the Beijing Synchrotron Radiation Facility.
Developing VUV spectroscopy for protein folding and material luminescence on beamline 4B8 at the Beijing Synchrotron Radiation Facility.
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DOI：
10.1107/s0909049509037236
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发表时间：
2009-11
影响因子：
2.5
通讯作者：
Ye Tao;Y. Huang;Zheng Gao;Hao Zhuang;A. Zhou;Yinglei Tan;Daowu Li;Shuaishuai Sun
中科院分区：
文献类型：
--
作者：
Ye Tao;Y. Huang;Zheng Gao;Hao Zhuang;A. Zhou;Yinglei Tan;Daowu Li;Shuaishuai Sun
新的4B8光束线提供波长范围从360到120纳米的UV-VUV光。它独特地实现了两种光谱测量：同步辐射圆二色光谱和VUV激发荧光光谱。前者主要用于蛋白质二级结构的研究，后者主要用于紫外激发发光材料的研究。荧光测量的远程访问已经实现，用户可以在线收集数据。除了稳态测量，荧光寿命测量已经建立了使用时域方法，而激光诱导的温度跳变正在开发中，用于蛋白质折叠动力学使用圆二色性作为探针。
The new 4B8 beamline provides UV-VUV light in the wavelength range from 360 to 120 nm. It uniquely enables two kinds of spectroscopy measurements: synchrotron radiation circular dichroism spectroscopy and VUV excited fluorescence spectroscopy. The former is mainly used in protein secondary structure studies, and the latter in VUV excited luminescent materials research. Remote access to fluorescence measurement has been realised and users can collect data online. Besides steady-state measurements, fluorescence lifetime measurements have been established using the time domain method, while a laser-induced temperature jump is under development for protein folding dynamics using circular dichroism as a probe.