Isolation and characterization of new microsatellite markers in mango (Mangifera indica)
Isolation and characterization of new microsatellite markers in mango (Mangifera indica)
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DOI：
10.1111/j.1471-8286.2004.00866.x
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发表时间：
2005-03-01
期刊：
影响因子：
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通讯作者：
Yonemori, K
中科院分区：
文献类型：
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作者：
Honsho, C;Nishiyama, K;Yonemori, K
从芒果（Mangifera indica）沿着的微卫星富集基因组文库中分离出6个微卫星位点，并利用其特异性引物对泰国主要栽培的36个芒果品种进行鉴定。观察到的和预期的杂合性范围从0到0.83和从0.29到0.73，分别。假定的等位基因的数量是2到6。6个等位基因中有3个的频率超过75%。这种高频率可能归因于品种起源的偏向。在供试的36个芒果品种中，有29个品种在6对引物中表现出独特的模式，而7个品种由于基因型相似性而无法鉴定。这表明微卫星标记在芒果品种鉴定中具有潜在的应用价值。
Six microsatellite loci that were isolated from a microsatellite-enriched genomic library of mango (Mangifera indica) along with their specific primer sets were each characterized by using 36 cultivars collected mainly in Thailand. The observed and expected heterozygosities ranged from 0 to 0.83 and from 0.29 to 0.73, respectively. The number of putative alleles are two to six. Three of the six alleles have frequencies of over 75%. The high frequency may be attributed to the bias in the origin of cultivars. Among 36 mango cultivars tested, 29 cultivars showed a unique pattern by six primer sets, whereas seven cultivars cannot be identified because of genotype similarities. This suggests the potentials for identification of mango cultivars by microsatellite markers.