Development of a multiplex real-time RT-PCR assay for simultaneous detection of dengue and chikungunya viruses
Development of a multiplex real-time RT-PCR assay for simultaneous detection of dengue and chikungunya viruses
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DOI：
10.1007/s00705-014-2217-x
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发表时间：
2015-01-01
影响因子：
2.7
通讯作者：
Shah, P. S.
中科院分区：
文献类型：
--
作者：
Cecilia, D.;Kakade, M.;Shah, P. S.
登革热和基孔肯雅病毒共同传播并导致感染，这些感染开始时症状相似，但进展到完全不同的结果。因此，需要一种能够区分两者的早期诊断测试。建立了一种可同时检测和定量所有登革热病毒（DENV）血清型和基孔肯雅病毒（CHIKV）RNA的一步法多重实时RT-PCR检测方法。与常规RT-PCR相比，DENV的灵敏度为100%，CHIKV为95.8%，而特异性为100%。检测限为1 ~ 50个空斑形成单位。该检测方法已成功用于鉴别诊断登革热和基孔肯雅病在浦那，其中病毒共同循环。
Dengue and chikungunya viruses co-circulate and cause infections that start with similar symptoms but progress to radically different outcomes. Therefore, an early diagnostic test that can differentiate between the two is needed. A single-step multiplex real-time RT-PCR assay was developed that can simultaneously detect and quantitate RNA of all dengue virus (DENV) serotypes and chikungunya virus (CHIKV). The sensitivity was 100 % for DENV and 95.8 % for CHIKV, whilst the specificity was 100 % for both viruses when compared with conventional RT-PCR. The detection limit ranged from 1 to 50 plaque-forming units. The assay was successfully used for differential diagnosis of dengue and chikungunya in Pune, where the viruses co-circulate.