Distribution of Leptin-Sensitive Cells in the Postnatal and Adult Mouse Brain
Distribution of Leptin-Sensitive Cells in the Postnatal and Adult Mouse Brain
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DOI：
10.1002/cne.22219
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发表时间：
2010-02-15
影响因子：
2.5
通讯作者：
Bouret, Sebastien G.
中科院分区：
文献类型：
--
作者：
Caron, Emilie;Sachot, Christelle;Bouret, Sebastien G.
瘦素在能量平衡和神经内分泌功能的调节中起着关键作用，越来越多的证据表明瘦素作用于大脑以介导许多这些作用。最近的数据也表明，瘦素影响出生后早期的大脑发育。在这里，我们研究了表达编码瘦素受体（LepRb）的长型mRNA的细胞在出生后和成年小鼠大脑中的分布，通过使用原位杂交。在成人和新生儿中，LepRb mRNA主要局限于已知控制能量平衡的区域。在下丘脑的弓状核、腹内侧核和背内侧核以及下丘脑外侧区中发现了标记细胞。在正中视前核和腹侧乳头体前核中也发现了重标记细胞，这两个核是已知控制生殖的下丘脑核。此外，在出生后和成年期，在下丘脑外的自主控制部位如孤束核中发现了清晰标记的细胞。重要的是，这种受体可以诱导细胞内信号传导，因为外周注射瘦素导致LepRb mRNA表达的大多数位点中的STAT 3磷酸化。LepRb mRNA在出生后小鼠大脑的某些区域也短暂升高，如皮质、海马和丘脑背侧核。两者合计，这些观察结果与瘦素在摄食和神经内分泌调节中的作用是一致的。他们还确定了LepRb mRNA在出生后早期表达的区域，并提出了瘦素在神经系统发育过程中的新作用。神经学比较杂志518. 459 - 476，2010. (C)2009 Wiley-Liss，Inc
Leptin plays a pivotal role in the regulation of energy homeostasis and neuroendocrine functions, and increasing evidence indicates that leptin acts on the brain to mediate many of these effects. Recent data have also suggested that leptin influences brain development during early postnatal life. Here we examined the distribution of cells that express mRNA encoding the long form of the leptin receptor (LepRb) in postnatal and adult mouse brains by using in situ hybridization. In both adults and neonates, LepRb mRNA was largely restricted to regions known to control energy balance. Labeled cells were found in the arcuate, ventromedial, and dorsomedial nuclei of the hypothalamus as well as in the lateral hypothalamic area Heavily labeled cells were also found in the median preoptic and ventral premammillary nuclei, two hypothalamic nuclei that are known to control reproduction Moreover, during postnatal and adult life, clearly labeled cells were found in extrahypothalamic autonomic control sites such as the nucleus of the tractus solitarius. Importantly, this receptor can induce intracellular signaling because peripheral injection of leptin caused STAT3 phosphorylation in most sites in which LepRb mRNA was expressed. LepRb mRNA was also transiently elevated in certain regions of the postnatal mouse brain, such as the cortex, hippocampus, and laterodorsal nucleus of the thalamus. Taken together, these observations are consistent with the proposed roles of leptin in feeding and neuroendocrine regulation. They also identify regions where LepRb mRNA is expressed during early postnatal life and suggest new roles for leptin in the nervous system during development. J. Comp. Neurol. 518. 459 - 476, 2010. (C) 2009 Wiley-Liss, Inc