Microtiter solid-phase radioimmunoassay for detection of human calicivirus in stools
Microtiter solid-phase radioimmunoassay for detection of human calicivirus in stools
复制标题
微量滴定固相放射免疫分析法检测粪便中人杯状病毒
DOI：
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发表时间：
1983
影响因子：
9.4
通讯作者：
T. Nakao
中科院分区：
文献类型：
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作者：
S. Nakata;S. Chiba;H. Terashima;Y. Sakuma;R. Kogasaka;T. Nakao
微量滴定固相放射免疫分析（RIA）被开发用于检测粪便样本中的人类杯状病毒。通过 RIA 检测了 78 份粪便样本。所有 17 份样本经电子显微镜 (EM) 检测呈人杯状病毒阳性，经放射免疫分析 (RIA) 检测也呈阳性。此外，在杯状病毒性胃肠炎疫情中获得的 21 份标本中，11 份放射免疫分析呈阳性，但电镜检测呈阴性。在 20 份 EM 检测轮状病毒呈阳性的样本和 20 份无病毒的非腹泻样本中，分别有 2 份和 1 份放射免疫分析呈阳性，但随后通过封闭试验显示为假阳性。人类和猫杯状病毒之间没有交叉反应。因此，该测试比 EM 更敏感，并且通过适当的封闭测试，对人类杯状病毒具有特异性。它对于筛查大量粪便标本可能特别有用。
A microtiter solid-phase radioimmunoassay (RIA) was developed for detection of human calicivirus in stool specimens. Seventy-eight stool specimens were tested by RIA. All 17 specimens positive for human calicivirus by electron microscopy (EM) were also positive by RIA. In addition, of 21 specimens obtained from an outbreak of caliciviral gastroenteritis, 11 were positive by RIA but negative by EM. Of 20 specimens positive for rotavirus by EM and 20 nondiarrheic specimens with no virus, 2 and 1, respectively, were positive by RIA but were subsequently shown to be falsely positive by a blocking test. There was no cross-reaction between human and feline caliciviruses. Thus, the test was more sensitive than EM and, with an appropriate blocking test, was specific for human calicivirus. It might be especially useful for screening large numbers of stool specimens.