Environmental virus detection associated with asymptomatic SARS-CoV-2-infected individuals with positive anal swabs
Environmental virus detection associated with asymptomatic SARS-CoV-2-infected individuals with positive anal swabs
复制标题
与肛门拭子呈阳性的无症状 SARS-CoV-2 感染者相关的环境病毒检测
DOI：
10.1016/j.scitotenv.2020.142289
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发表时间：
2021-01-20
影响因子：
9.8
通讯作者：
Sun, Hong
中科院分区：
文献类型：
--
作者：
Huang, Ying;Ding, Zhen;Sun, Hong
在抗击中国暴发的新冠肺炎中，我们救治了一些无症状的感染者。这项研究旨在检测这些无症状感染者的生物和环境样本中的病原体，并分析它们之间的关系。采用横断面研究设计，采集了南京市第二医院隔离病房收治的19例患者的生物样品和环境样品。生物样本包括唾液、咽拭子、血液、肛门拭子和呼出的呼吸冷凝物。病房环境中的拭子样本包括内部口罩、外部口罩、手掌拭子、床边扶手、床头柜、手机屏幕、厕所手机架、马桶垫和马桶盖。我们还从公共区域获得了一些样本。用逆转录聚合酶链式反应检测病原体，用胶体金检测抗体。结果，19名无症状感染者参加了调查，其中8名病原体阳性，11名抗体阳性。从96个环境样本中检测出3个阳性样本，分别来自手机表面、手机架和床边扶手。在本次工作中，呼出的呼气冷凝液中未检测到阳性样本。所有在环境中发现病原体的患者都有阳性的肛拭子。阳性的肛拭子和阳性的环境样本之间有统计学上的联系。从无症状感染患者周围采集的阳性样本与阳性肛拭子之间的关联表明，患者可能会在更长的时间内分泌病毒。(C)2020爱思唯尔B.V.保留所有权利。
In the fight against the outbreak of COVID-19 in China, we treated some asymptomatic infected individuals. This study aimed to detect pathogens in biological and environmental samples of these asymptomatic infected individuals and analyse their association. Using a cross-sectional study design, we collected biological and environmental samples from 19 patients treated in the isolation ward of Nanjing No.2 Hospital. Biological samples included saliva, pharyngeal swabs, blood, anal swabs, and exhaled breath condensate. Swab samples from the ward environment included inside masks, outside masks, palm swabs, bedside handrails, bedside tables, cell phone screens, toilet cell phone shelves, toilet pads and toilet lids. We also obtained some samples from public areas. We used RT-PCR to detect pathogens and colloidal gold to detect antibodies. As results, 19 asymptomatic infected individuals participated in the survey, with 8 positives for pathogens and 11 positives only for antibodies. Three positive samples were detected from among 96 environmental samples, respectively, from a cell phone surface, a cell phone shelf and a bedside handrail. No positive samples were detected in the exhaled breath condensate in this work. All patients identified pathogens in the environment had positive anal swabs. There was a statistical association between positive anal swabs and positive environmental samples. The association of positive samples from the surrounding of asymptomatically infected patients with positive anal swabs suggested that patients might secrete the virus for a more extended period. (C) 2020 Elsevier B.V. All rights reserved.