Plasma levels and adipose tissue messenger ribonucleic acid expression of retinol-binding protein 4 are reduced during calorie restriction in obese subjects but are not related to diet-induced changes in insulin sensitivity
Plasma levels and adipose tissue messenger ribonucleic acid expression of retinol-binding protein 4 are reduced during calorie restriction in obese subjects but are not related to diet-induced changes in insulin sensitivity
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DOI：
10.1210/jc.2006-2668
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发表时间：
2007-06-01
影响因子：
5.8
通讯作者：
Langin, Dominique
中科院分区：
文献类型：
--
作者：
Vitkova, Michaela;Klimcakova, Eva;Langin, Dominique
内容：Retinol-binding protein 4（RBP 4）可能在胰岛素抵抗的发展中发挥作用。目的：我们研究了饮食诱导的体重减轻过程中RBP 4脂肪组织mRNA表达和血浆水平是否与胰岛素敏感性相关。设计、设置、患者和干预：肥胖妇女遵循饮食干预，包括4周的极低热量饮食（VLCD），2个月的低热量饮食，主要观察指标：在每一阶段治疗前和治疗结束时进行临床观察。用正常血糖高胰岛素钳夹法评估胰岛素敏感性。结果：VLCD期间体重和脂肪量下降，WM期间体重和脂肪量稳定。葡萄糖处置率在VLCD期间增加，此后保持升高。血浆RBP 4水平下降后VLCD，虽然在随后的阶段增加，仍然低于prediet值。脂肪组织mRNA水平在VLCD后减少，在低热量饮食和WM期间增加，达到基础值。基础RBP 4水平或饮食引起的变化RBP 4瘦妇女和两组肥胖妇女与高和低胰岛素sensitivity.Conclusions：严重的热量限制促进减少脂肪组织和血浆水平的RBP 4。这项研究没有提供证据证明RBP 4在调节饮食诱导的胰岛素敏感性变化中的作用。
Context: Retinol-binding protein 4 (RBP4) may play a role in the development of insulin resistance.Objective: We investigated whether RBP4 adipose tissue mRNA expression and plasma level are related to insulin sensitivity during a diet-induced weight loss.Design, Setting, Patients, and Intervention: Obese women followed a dietary intervention composed of a 4-wk very low-calorie diet (VLCD), a 2-month low-calorie diet, and 3-4 months of a weight maintenance (WM) phase.Main Outcome Measures: Clinical investigation was performed before and at the end of each phase. Insulin sensitivity was assessed with the euglycemic hyperinsulinemic clamp. Adipose tissue mRNA and plasma levels of RBP4 were determined using reverse transcription-quantitative PCR and ELISA, respectively.Results: Weight and fat mass decreased during VLCD and were stabilized during WM. Glucose disposal rate increased during VLCD and remained elevated thereafter. Plasma levels of RBP4 decreased after VLCD and, although increasing at subsequent phases, remained lower than prediet values. Adipose tissue mRNA levels were diminished after VLCD, and increased during low-calorie diet and WM to reach basal values. Basal RBP4 levels or diet-induced variations of RBP4 were not different in lean women and two groups of obese women with high-and low-insulin sensitivity.Conclusions: Severe calorie restriction promotes a reduction in adipose tissue and plasma levels of RBP4. The study does not bring evidence for a role for RBP4 in the regulation of diet-induced changes in insulin sensitivity.