The expression and significance of leukemia inhibitory factor, interleukin-6 and vascular endothelial growth factor in Chinese patients with endometriosis
The expression and significance of leukemia inhibitory factor, interleukin-6 and vascular endothelial growth factor in Chinese patients with endometriosis
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DOI：
10.1007/s00404-021-05980-5
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发表时间：
2021-02
影响因子：
2.6
通讯作者：
Cui Li;Honglian Zhao;Yujuan Li;Yu-Ying Zhang;Hong-Yun Liu;F. Feng;Hua Yan
中科院分区：
文献类型：
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作者：
Cui Li;Honglian Zhao;Yujuan Li;Yu-Ying Zhang;Hong-Yun Liu;F. Feng;Hua Yan
目的观察异位内膜异位症患者血液、腹腔液、异位内膜组织、异位内膜基质细胞中白血病抑制因子(LIF)、白介素6(IL-6)、血管内皮生长因子(VEGF)的水平，比较异位子宫内膜异位症患者与非异位内膜患者的IL-6、IL-6、血管内皮生长因子的表达。检测两组患者血和腹腔液中LIF、IL-6和VEGF的水平。检测观察组患者异位内膜组织和自体在位内膜组织、异位内膜间质细胞和自体在位内膜间质细胞中LIF、IL-6和VEGF的水平。结果观察组大鼠血和腹腔液中LIF、IL-6和VEGF值均高于对照组(P<； )，且观察组腹腔液中LIF、IL-6和VEGF值明显高于血中(P<； )。观察组异位内膜组织中LIF-m RNA和IL-6 m RNA的表达水平高于自身配对的在位内膜组织(P<； 0.0 5)，而异位内膜组织中VEGF m RNA的表达水平低于自身配对的在位内膜组织(P<； 0.0 5)。此外，观察组异位内膜基质细胞中LIF、IL-6和VEGFmRNA的表达水平均高于自体配对的在位内膜基质细胞(P<； )。经LIF、IL-6和LIF + IL-6分别刺激异位内膜间质细胞后，培养上清液中的 水平均显著高于空白对照组(P<；VEGF0.05)。结论内异症患者血和腹腔液中的LIF、IL-6和VEGF3种因子水平均升高，且异位内异症间质细胞中的LIF和IL-6水平升高可能起协同作用，可能参与了子宫内膜异位症的发生发展过程。
ObjectiveTo observe the levels of leukemia inhibitory factor (LIF), interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) in blood, peritoneal fluid, ectopic endometrial tissue, and ectopic endometrial stromal cells of patients with endometriosis, and to compare expression of IL-6, LIF and VEGF expression between endometriotic and non-endometriotic patients underwent laparoscopic surgery.MethodsThirty-one patients who underwent laparoscopic surgery for endometriosis in our hospital from January 2018 to January 2020 were included in the observation group, and 32 patients who underwent laparoscopic surgery for uterine fibroids, ovarian serous cystadenoma, and ovarian teratomas, were included in the control group. The levels of LIF, IL-6 and VEGF in the blood and peritoneal fluid of the two groups of patients were detected. The levels of LIF, IL-6 and VEGF in ectopic endometrial tissue and self-paired eutopic endometrial tissue, ectopic endometrial stromal cells and self-paired eutopic endometrial stromal cells of patients in the observation group were detected. After treating the primary cultured ectopic endometrial stromal cells with LIF and IL-6 alone or in combination, the changes of VEGF mRNA of ectopic endometrial stromal cells and the VEGF levels in the supernatant were observed.ResultsThe levels of LIF, IL-6 and VEGF in the blood and peritoneal fluid of the observation group were all higher than those of the control group (P< 0.05), and the levels of LIF, IL-6 and VEGF in the peritoneal fluid of the observation group were significantly higher than those in the blood (P< 0.05). In the observation group, the expression levels of LIF-mRNA and IL-6 mRNA in the ectopic endometrial tissue were higher than those in the self-paired eutopic endometrial tissues (P< 0.05), while the expression level of VEGF mRNA in the ectopic endometrial tissues was lower than that in the self-paired eutopic endometrial tissues (P< 0.05). Besides, the mRNA expression levels of LIF, IL-6 and VEGF in ectopic endometrial stromal cells of the observation group were all higher than those in the self-paired eutopic endometrial stromal cells (P< 0.05). After stimulating ectopic endometrial stromal cells with LIF, IL-6 and LIF + IL-6, respectively, the VEGF levels in the supernatant were all significantly higher than that in the blank control group (P< 0.05).ConclusionThe LIF, IL-6 and VEGF levels in blood and peritoneal fluid were increased in patients with endometriosis, and increased LIF and IL-6 in ectopic endometriosis stromal cells can play a synergistic role in increasing the VEGF levels, which may be involved in the occurrence and development of endometriosis.