A novel neuropeptide in suppressing luteinizing hormone release in goldfish, Carassius auratus
A novel neuropeptide in suppressing luteinizing hormone release in goldfish, Carassius auratus
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一种抑制金鱼黄体生成素释放的新型神经肽，鲫鱼
DOI：
10.1016/j.mce.2013.04.008
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发表时间：
2013-07-15
影响因子：
4.1
通讯作者：
Cheng, Christopher H. K.
中科院分区：
文献类型：
--
作者：
Liu, Yun;Li, Shuisheng;Cheng, Christopher H. K.
鱼类生殖轴受多种神经内分泌因子的调控。然而，抑制这一轴的因素在很大程度上是不确定的。在这项研究中，我们描述了一种新的神经肽来源于spexin前体作为一个负性因素，抑制生殖轴的硬骨鱼。spexin前体的cDNA序列已经从斑马鱼和金鱼中克隆出来。C-末端酰胺化的14-aa成熟肽（spexin-14 a：NWTPQAMLYLKGTQ-NH 2）可通过加工两种物种中的spexin前体而产生。Spexin主要在斑马鱼的脑和卵巢中表达，spexin-14 a-ir细胞分布于金鱼的多个脑区。在功能上，金鱼spexin-14 a可显著抑制培养的金鱼垂体细胞的促黄体生成素（LH）释放。腹腔注射spexin-14 a可有效抑制血清LH水平。spexin在繁殖季节的mRNA表达量较低，下丘脑spexin的表达受性腺激素的调节。这些结果构成了关于spexin在硬骨鱼生殖轴负调控中的新作用的第一份报告（c）2013 Elsevier爱尔兰Ltd.保留所有权利。
The fish reproductive axis is regulated by many neuroendocrine factors. However, factors involved in the suppression of this axis are largely uncharacterized. In this study, we describe a novel neuropeptide derived from the spexin precursor acting as a negative factor to suppress the reproductive axis in teleost. The cDNA sequences of the spexin precursors have been cloned from both zebrafish and goldfish. A 14-aa mature peptide with the C-terminal amidated (spexin-14a: NWTPQAMLYLKGTQ-NH2) is conceivably generated by processing of the spexin precursors in both species. Spexin is mainly expressed in the brain and ovary of zebrafish and spexin-14a-ir cells are located in several brain regions of goldfish. Functionally, goldfish spexin-14a could significantly suppress luteinizing hormone (LH) release in cultured goldfish pituitary cells. Moreover, intraperitoneal injection of spexin-14a could effectively suppress serum LH level. The mRNA expression of spexin is lower in the breeding season and hypothalamic expression of spexin is regulated by gonadal hormones. These results constitute the first report on the novel role of spexin in the negative regulation of the reproductive axis in teleost (c) 2013 Elsevier Ireland Ltd. All rights reserved.