Reliable microfluidic on-chip incubation of droplets in delay-lines.
Reliable microfluidic on-chip incubation of droplets in delay-lines.
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DOI：
10.1039/b816049j
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发表时间：
2009-05-21
期刊：
影响因子：
6.1
通讯作者：
Griffiths AD
中科院分区：
文献类型：
--
作者：
Frenz L;Blank K;Brouzes E;Griffiths AD
与液滴的产生、融合和分选一起，液滴的孵育是基于液滴的微流体分析的最重要和最基本的操作之一。这份手稿涉及延迟线的发展，这是必要的，以允许精确的时间段内孵育的反应。我们分析了与创建延迟线孵育在分钟到小时的时间范围内，这引起的背压和分散在孵育时间由于液滴通过延迟线的速度不相等的问题。我们描述了延迟线系统，解决这些问题，并证明了它们的使用来测量反应动力学在几分钟内的液滴。
Together with droplet creation, fusion and sorting, the incubation of droplets is one of the most important and essential operations for droplet-based microfluidic assays. This manuscript concerns the development of delay-lines, which are necessary to allow incubation of reactions for precise time periods. We analyze the problems associated with creating delay-lines for incubation in the minute to hour time range, which arise from back-pressure and from the dispersion in the incubation time due to the unequal speeds with which droplets pass through the delay-line. We describe delay-line systems which resolve these problems and demonstrate their use to measure reaction kinetics over several minutes in droplets.