pen repeat sequences are GGN clusters and encode a glycine-rich domain in a Drosophila cDNA homologous to the rat helix destabilizing protein.
pen repeat sequences are GGN clusters and encode a glycine-rich domain in a Drosophila cDNA homologous to the rat helix destabilizing protein.
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笔重复序列是 GGN 簇，编码果蝇 cDNA 中与大鼠螺旋不稳定蛋白同源的富含甘氨酸的结构域。
DOI：
10.1073/pnas.84.7.1819
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发表时间：
1987
影响因子：
11.1
通讯作者：
I. Dawid
中科院分区：
文献类型：
--
作者：
S. Haynes;M. Rebbert;B. Mozer;F. Forquignon;I. Dawid
已经分离出几个含有pen重复序列的cDNA克隆并进行了测序; pen由GGN三联体簇组成，其中N可以是任何核苷酸。在长开放阅读框内发现了一些笔重复序列，其中它们编码寡聚甘氨酸延伸。对于其中一个克隆，整个开放阅读框的推导氨基酸序列，特别是在富含甘氨酸的结构域之前的区域中，显示出与大鼠螺旋去稳定化蛋白的强同源性[Cobianchi，F.，SenGupta，D. N.，兹穆兹卡，B。Z. &威尔逊，S。H.（1986）J.Biol.Chem.261，3536-3543]。大鼠蛋白和其他生物中的同源物是单链核酸结合蛋白，其中一些是异质核核糖核蛋白颗粒的主要成分。我们建议，我们已经克隆了一个cDNA编码的果蝇单链核酸结合蛋白。
Several cDNA clones that contain the pen repeat have been isolated and sequenced; pen consists of clusters of GGN triplets, where N can be any nucleotide. Some of the pen repeat sequences are found within long open reading frames in which they encode oligoglycine stretches. For one of the clones, the deduced amino acid sequence of the entire open reading frame, especially in the region preceding the glycine-rich domain, shows strong homology to the rat helix destabilizing protein [Cobianchi, F., SenGupta, D. N., Zmudzka, B. Z. & Wilson, S. H. (1986) J. Biol. Chem. 261, 3536-3543]. The rat protein and homologs in other organisms are single-stranded nucleic acid binding proteins, some of which are major components of heterogeneous nuclear ribonucleoprotein particles. We suggest that we have cloned a cDNA encoding a Drosophila single-stranded nucleic acid binding protein.