Cometabolic degradation of dibenzofuran and dibenzothiophene by a newly isolated carbazole-degrading Sphingomonas sp strain
Cometabolic degradation of dibenzofuran and dibenzothiophene by a newly isolated carbazole-degrading Sphingomonas sp strain
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新分离的咔唑降解鞘氨醇单胞菌菌株对二苯并呋喃和二苯并噻吩的共代谢降解
DOI：
10.1128/aem.02704-06
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发表时间：
2007-05-01
影响因子：
4.4
通讯作者：
Xu, Ping
中科院分区：
文献类型：
--
作者：
Gai, Zhonghui;Yu, Bo;Xu, Ping
从石油污染土壤中富集分离到一株咔唑利用菌。该菌株命名为鞘氨醇单胞菌XLDN 2 -5，能够利用咔唑（CA）作为唯一的碳源、氮源和能量源。菌株XLDN 2 -5经洗涤后能降解二苯并呋喃（DBF）和二苯并噻吩（DBT）。对代谢物的检查表明，XLDN 2 -5通过角双氧合途径将DBF降解为2-羟基-6-（2-羟基苯基）-6-氧代-2，4-己二烯酸，随后降解为水杨酸。与DBF相比，菌株XLDN 2 -5可以通过开环和磺基氧化途径转化DBT。鞘氨醇单胞菌XLDN 2 -5在以CA为底物的生长体系中能共代谢DBF和DBT。孵育40小时后，90%的DBT被转化，CA和DBF被完全去除。这些结果表明，菌株XLDN 2 -5可能是有用的，在生物修复的环境污染的这些化合物。
A carbazole-utilizing bacterium was isolated by enrichment from petroleum-contaminated soil. The isolate, designated Sphingomonas sp. strain XLDN2-5, could utilize carbazole (CA) as the sole source of carbon, nitrogen, and energy. Washed cells of strain XLDN2-5 were shown to be capable of degrading dibenzofuran (DBF) and dibenzothiophene (DBT). Examination of metabolites suggested that XLDN2-5 degraded DBF to 2-hydroxy-6-(2-hydroxyphenyl)-6-oxo-2,4-hexadienic acid and subsequently to salicylic acid through the angular dioxygenation pathway. In contrast to DBF, strain XLDN2-5 could transform DBT through the ring cleavage and sulfoxidation pathways. Sphingomonas sp. strain XLDN2-5 could cometabolically degrade DBF and DBT in the growing system using CA as a substrate. After 40 h of incubation, 90% of DBT was transformed, and CA and DBF were completely removed. These results suggested that strain XLDN2-5 might be useful in the bioremediation of environments contaminated by these compounds.