Construction and testing of a microsatellite database containing more than 500 tomato varieties
Construction and testing of a microsatellite database containing more than 500 tomato varieties
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DOI：
10.1007/s00122-002-1038-6
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发表时间：
2002-11-01
影响因子：
5.4
通讯作者：
Vosman, B
中科院分区：
文献类型：
--
作者：
Bredemeijer, GMM;Cooke, RJ;Vosman, B
本研究的目的是评价序列标记微卫星位点（STMS）标记在番茄品种鉴定和鉴别中的适用性。为此，利用20对STMS引物对构建了一个包含欧洲最常见番茄品种（bbb500）分子描述的数据库。这个数据库是由五个欧洲实验室组成的联盟建立和测试的，每个实验室使用不同的STMS检测系统。通过这种方式，可以证明STMS标记和数据库适合在网络活动中使用，在这些活动中，正在不断地用来自不同实验室的数据建立一个共同的数据库。番茄微卫星多态性相对较低。每个位点的等位基因数为2 ~ 8个，平均为4.7个。然而，90%以上的品种具有不同的微卫星剖面。“盲测”表明，一般情况下，使用20个标记和数据库对同质品种识别未知样品（或检测最相似的品种）相对容易，但在使用6个个体的池时，对异质品种识别的确定性较低。
The aim of this study was to evaluate the suitability of sequence tagged microsatellite site (STMS) markers for varietal identification and discrimination in tomato. For this purpose, a set of 20 STMS primer pairs was used to construct a database containing the molecular description of the most common varieties (>500) of tomato grown in Europe. The database was built and tested by a consortium of five European laboratories each using a different STMS detection system. In this way, it could be demonstrated that the STMS markers and database were suitable for use in network activities where a common database is being established on a continuing basis with data from different laboratories.Microsatellite polymorphism in tomato was found to be relatively low. The number of alleles per locus ranged from 2 to 8 with an average of 4.7 alleles per locus. Nevertheless, more than 90% of the varieties had different microsatellite profiles. A "blind testing" exercise showed that in general, identification of unknown samples (or detecting the most similar variety) with the 20 markers and the database was relatively easy for homogeneous varieties but less certain with heterogeneous varieties when using pools of 6 individuals.