Interleukin-9 blockage reduces early hepatic granuloma formation and fibrosis during Schistosoma japonicum infection in mice
Interleukin-9 blockage reduces early hepatic granuloma formation and fibrosis during Schistosoma japonicum infection in mice
复制标题
白细胞介素 9 阻断可减少小鼠日本血吸虫感染期间的早期肝肉芽肿形成和纤维化
DOI：
10.1111/imm.13111
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发表时间：
2019
期刊：
影响因子：
6.4
通讯作者：
Xia Chaoming
中科院分区：
文献类型：
--
作者：
Zhan Tingzheng;Ma Huihui;Jiang Suqin;Zhong Zirong;Wang Xiaoli;Li Chunxiang;Yu Dan;Liu Lei;Xu Jing;Xia Chaoming
血吸虫诱导的肝纤维化是由一个复杂的细胞因子网络调控的。T辅助9型细胞（Th9）是一类新型的T辅助细胞，主要分泌特异性细胞因子白细胞介素- 9 （IL - 9）。在四氯化碳引起的慢性乙型肝炎患者和小鼠模型中，白细胞介素- 9已被证明有助于肝纤维化。然而，IL - 9在血吸虫病纤维化中的作用尚不清楚。在这项研究中，我们通过体内和体外研究探讨了IL - 9在血吸虫病中的作用。他们的体内研究发现，IL - 9的中和减少了肝脏肉芽肿炎症和寄生虫卵周围的胶原沉积。他们的体外研究发现，用IL - 9处理原代肝星状细胞可诱导胶原蛋白和α -平滑肌肌动蛋白显著增加。此外，我们还描述了IL - 9和IL - 4在日本血吸虫感染小鼠体内的动态和相关性。我们发现IL - 9可能比IL - 4出现得更快、水平更高。因此，我们的研究结果表明，IL - 9可能在血吸虫病早期肝纤维化的调控中发挥作用，并成为调控日本血吸虫引起的肝纤维化的一种有前景的途径。
Hepatic fibrosis induced by schistosomes is regulated by a complex network of cytokines. T helper type 9 (Th9) cells are a new type of effector T helper cells, which mainly secrete the specific cytokine interleukin‐9 (IL‐9). Interleukin‐9 has been shown to contribute to liver fibrosis in patients with chronic hepatitis B and in a mouse model due to carbon tetrachloride. However, the role of IL‐9 in schistosomiasis fibrosis remains unknown. In this study, we investigated the roles of IL‐9 in schistosomiasis throughin vivoandin vitrostudies. Thein vivostudies found that neutralization of IL‐9 reduced liver granulomatous inflammation and collagen deposition around parasite eggs. Thein vitrostudies found that the treatment of primary hepatic stellate cells with IL‐9 induced a significant increase of collagen andα‐smooth‐muscle actin. Moreover, we also described the dynamics and relevance of IL‐9 and IL‐4 in mice infected withSchistosoma japonicum. We found that IL‐9 might appear more quickly and at higher levels than IL‐4. Hence, our findings indicated that IL‐9 might play a role in regulating hepatic fibrosis in early‐stage schistosomiasis and become a promising approach for regulating hepatic fibrosis caused byS.japonicum.