Biofilm Formation by Staphylococcus aureus Isolates from Skin and Soft Tissue Infections
Biofilm Formation by Staphylococcus aureus Isolates from Skin and Soft Tissue Infections
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DOI：
10.1007/s00284-014-0770-x
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发表时间：
2015-05-01
影响因子：
2.6
通讯作者：
Jin, Tao
中科院分区：
文献类型：
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作者：
Kwiecinski, Jakub;Kahlmeter, Gunnar;Jin, Tao
由金黄色葡萄球菌引起的许多疾病与生物膜形成有关。然而，S.尚未研究来自皮肤和软组织感染的金黄色葡萄球菌分离物形成生物膜。我们测试了160株来自各种皮肤感染患者的菌株在不同生长培养基中的生物膜形成能力。所有的菌株形成生物膜，其程度取决于生长培养基的类型。最厚的生物膜形成时，血浆和葡萄糖都存在于肉汤中，在这种情况下，S。金黄色葡萄球菌将宿主纤维蛋白掺入生物膜的基质中。不同类型皮肤感染的分离株之间的生物膜形成没有差异，除了糖尿病伤口分离株的生物膜形成特别好，以及脓疱病分离株的生物膜形成较弱。总之，生物膜形成是S的普遍行为。金黄色葡萄球菌从皮肤感染中分离出来。在某些情况下，例如在糖尿病伤口中，特别强烈的生物膜形成最有可能导致感染的慢性和复发性。此外，作为S。金黄色葡萄球菌显然使用宿主纤维蛋白作为生物膜结构的一部分，我们建议在体外生物膜研究中应更频繁地包括血浆。
Many diseases caused by Staphylococcus aureus are associated with biofilm formation. However, the ability of S. aureus isolates from skin and soft tissue infections to form biofilms has not yet been investigated. We tested 160 isolates from patients with various skin infections for biofilm-forming capacity in different growth media. All the isolates formed biofilms, the extent of which depended on the type of growth medium. The thickest biofilms were formed when both plasma and glucose were present in the broth; in this case, S. aureus incorporated host fibrin into the biofilm's matrix. There were no differences in the biofilm formation between isolates from different types of skin infections, except for a particularly good biofilm formation by isolates from diabetic wounds and a weaker biofilm formation by isolates from impetigo. In conclusion, biofilm formation is a universal behavior of S. aureus isolates from skin infections. In some cases, such as in diabetic wounds, a particularly strong biofilm formation most likely contributes to the chronic and recurrent character of the infection. Additionally, as S. aureus apparently uses host fibrin as part of the biofilm structure, we suggest that plasma should be included more frequently in in vitro biofilm studies.