Membrane-associated DNA Transport Machines
Membrane-associated DNA Transport Machines
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DOI：
10.1101/cshperspect.a000406
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发表时间：
2010-07-01
影响因子：
7.2
通讯作者：
Dubnau, David
中科院分区：
文献类型：
--
作者：
Burton, Briana;Dubnau, David
DNA泵在细菌细胞分裂期间以及通过接合和转化转移遗传物质期间发挥重要作用。FtsK/SpoIIIE蛋白进行双链DNA的易位，以确保在细胞分裂期间染色体完全分离。相反，介导接合和遗传转化的复杂分子机器驱动单链DNA的运输。转化机器也处理这种内化的DNA，并在摄取期间和之后介导其与常驻染色体的重组，而接合装置在转移之前处理DNA。本文综述了这三种类型的DNA泵，注意什么是了解他们的分子机制，他们的能量和他们的细胞定位。
DNA pumps play important roles in bacteria during cell division and during the transfer of genetic material by conjugation and transformation. The FtsK/SpoIIIE proteins carry out the translocation of double-stranded DNA to ensure complete chromosome segregation during cell division. In contrast, the complex molecular machines that mediate conjugation and genetic transformation drive the transport of single stranded DNA. The transformation machine also processes this internalized DNA and mediates its recombination with the resident chromosome during and after uptake, whereas the conjugation apparatus processes DNA before transfer. This article reviews these three types of DNA pumps, with attention to what is understood of their molecular mechanisms, their energetics and their cellular localizations.