Genome-wide identification of polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori.
Genome-wide identification of polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori.
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DOI：
10.1371/journal.pone.0034330
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Kusakabe T
中科院分区：
文献类型：
--
作者：
Li Z;Cheng D;Mon H;Tatsuke T;Zhu L;Xu J;Lee JM;Xia Q;Kusakabe T
Polycomb group （PcG）蛋白是进化上保守的染色质修饰因子，并在三种多聚体复合物中共同作用，Polycomb抑制复合物1 (PRC1)， Polycomb抑制复合物2 （PRC2）和多同源性抑制复合物（PhoRC），以抑制靶基因的转录。本研究通过敲低BmSCE、BmESC、BmPHO和BmSCM基因，在家蚕全新中心着丝粒中鉴定了Polycomb靶基因。结果，敲除4个PcG基因后，29个基因的表达上调。特别是，BmPho靶点（524个靶点中有331个）和BmScm靶点（532个靶点中有331个）之间存在显著的重叠，其中190个基因作为调控因子在发育中发挥重要作用。我们还发现，BmPho和BmScm可以与本研究中检测的其他Polycomb成分相互作用。进一步详细分析发现，含有锌指结构域的BmPho的c端参与了BmPho与BmScm的相互作用。此外，BmPho中的锌指结构域有助于其抑制功能，并且BmScm的异位过表达能够促进Gal4-Pho融合包括BmScm相互作用结构域的转录抑制。BmPho表达缺失导致BmScm重新定位到细胞质中。总之，我们提供了BmPho和BmScm之间功能联系的证据，并提出了两种与polycomb相关的抑制机制，只需要与BmScm相关的BmPho或一整套PcG复合物。
Polycomb group (PcG) proteins are evolutionarily conserved chromatin modifiers and act together in three multimeric complexes, Polycomb repressive complex 1 (PRC1), Polycomb repressive complex 2 (PRC2), and Pleiohomeotic repressive complex (PhoRC), to repress transcription of the target genes. Here, we identified Polycomb target genes in Bombyx mori with holocentric centromere using genome-wide expression screening based on the knockdown of BmSCE, BmESC, BmPHO, or BmSCM gene, which represent the distinct complexes. As a result, the expressions of 29 genes were up-regulated after knocking down 4 PcG genes. Particularly, there is a significant overlap between targets of BmPho (331 out of 524) and BmScm (331 out of 532), and among these, 190 genes function as regulator factors playing important roles in development. We also found that BmPho, as well as BmScm, can interact with other Polycomb components examined in this study. Further detailed analysis revealed that the C-terminus of BmPho containing zinc finger domain is involved in the interaction between BmPho and BmScm. Moreover, the zinc finger domain in BmPho contributes to its inhibitory function and ectopic overexpression of BmScm is able to promote transcriptional repression by Gal4-Pho fusions including BmScm-interacting domain. Loss of BmPho expression causes relocalization of BmScm into the cytoplasm. Collectively, we provide evidence of a functional link between BmPho and BmScm, and propose two Polycomb-related repression mechanisms requiring only BmPho associated with BmScm or a whole set of PcG complexes.
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影响因子：
16
作者：
Kanhere A;Viiri K;Araújo CC;Rasaiyaah J;Bouwman RD;Whyte WA;Pereira CF;Brookes E;Walker K;Bell GW;Pombo A;Fisher AG;Young RA;Jenner RG
通讯作者：
Jenner RG
影响因子：
7.8
作者：
Buchenau, P;Hodgson, J;Strutt, H;Arndt-Jovin, DJ
通讯作者：
Arndt-Jovin, DJ
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
7.7
作者：
Grimm, Clemens;de Ayala Alonso, Andres Gaytan;Mueller, Christoph W.
通讯作者：
Mueller, Christoph W.
影响因子：
10.5
作者：
Leeb, Martin;Pasini, Diego;Wutz, Anton
通讯作者：
Wutz, Anton