Purification and structural characterization of a new water-soluble neutral polysaccharide GLP-F1-1 from Ganoderma lucidum
Purification and structural characterization of a new water-soluble neutral polysaccharide GLP-F1-1 from Ganoderma lucidum
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DOI：
10.1016/j.ijbiomac.2010.10.015
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发表时间：
2011-01-01
影响因子：
8.2
通讯作者：
Wang, Zhou
中科院分区：
文献类型：
--
作者：
Huang, Sheng-quan;Li, Jin-wei;Wang, Zhou
采用DEAE Sepharose Fast Flow和Sephacryl S-500高分辨色谱从灵芝子实体中分离得到水溶性中性多糖(GLP-F1-1)。中性多糖的平均分子量(M(w))约为2.5 x 10(6) kDa。 GC分析表明该多糖主要由葡萄糖和半乳糖组成，摩尔比为34:1。 (1)H和(13)C NMR光谱结合GC-MS技术表明，新多糖具有1,4-二取代-β-吡喃葡萄糖和1,4,6-三取代-β-吡喃葡萄糖基的主链，而支链主要由1,6-二取代-β-吡喃葡萄糖基和1,4-二取代-β-吡喃半乳糖基残基组成。 (C) 2010 Elsevier B.V. 保留所有权利。
A water-soluble neutral polysaccharide (GLP-F1-1) was isolated from the fruiting bodies of Ganoderma lucidum by DEAE Sepharose Fast Flow and Sephacryl S-500 High Resolution Chromatography. The neutral polysaccharide had an average molecular weight (M(w)) of approximately 2.5 x 10(6) kDa. GC analysis showed that this polysaccharide was mainly composed of glucose and galactose in the molar ratio of 34:1. (1)H and (13)C NMR spectroscopy in combination with GC-MS technique indicated that the new polysaccharide had a backbone chain of 1,4-disubstituted-beta-glucoseopyranose and 1,4,6-trisubstituted-beta-glucoseopyranosyl, while the branched chains were mainly composed of 1,6-disubstituted-beta-glucopyranosyl and 1,4-disubstituted-beta-galactoseopyranosyl residues. (C) 2010 Elsevier B.V. All rights reserved.