Structure-based redesign of Fe(<scp>ii</scp>)/2-oxoglutarate-dependent oxygenase AndA to catalyze spiro-ring formation
Structure-based redesign of Fe(<scp>ii</scp>)/2-oxoglutarate-dependent oxygenase AndA to catalyze spiro-ring formation
复制标题
基于结构的 Fe(<scp>ii</scp>)/2-酮戊二酸依赖性加氧酶 AndA 催化螺环形成的重新设计
DOI：
10.1039/d2cc00736c
复制
发表时间：
2022
影响因子：
4.9
通讯作者：
Abe Ikuro
中科院分区：
文献类型：
--
作者：
Mori Takahiro;Nakashima Yu;Chen Heping;Hoshino Shotaro;Mitsuhashi Takaaki;Abe Ikuro
对Fe（II）/2-酮戊二酸依赖性加氧酶AndA进行了基于结构和机制的重新设计。AndA的功能被扩展到催化异构化反应生成螺环。重新设计的AndA变体通过两次和三次连续的氧化反应产生了两种非天然的新型含螺环的化合物。
Structure- and mechanism-based redesign of the Fe(II)/2-oxoglutarate-dependent oxygenase AndA was performed. The function of AndA was expanded to catalyze a spiro-ring formation reaction from an isomerization reaction. The redesigned AndA variants produced two unnatural novel spiro-ring containing compounds through two and three consecutive oxidation reactions.