Synthesis of chromogranin A, dopamine beta-hydroxylase, and chromaffin vesicles.
Synthesis of chromogranin A, dopamine beta-hydroxylase, and chromaffin vesicles.
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嗜铬粒蛋白 A、多巴胺 β-羟化酶和嗜铬小泡的合成。
DOI：
10.1152/ajpcell.1990.259.1.c161
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Kirshner,N
中科院分区：
文献类型：
--
作者：
Corcoran,JJ;Kirshner,N
原代培养的牛肾上腺髓细胞合成嗜铬粒蛋白A （CgA）和多巴胺β -羟化酶（DBH），并将其整合到嗜铬蛋白囊泡中。在蛋氨酸浓度为12.5、25和50微米时，L-[35S]蛋氨酸在CgA、DBH和总蛋白中的掺入在8小时内近似成线性关系。新合成的CgA和DBH最初被纳入低浮力密度的囊泡中，在24小时内成熟为具有较大浮力密度的染色质囊泡。大约10%的新合成的CgA在形成后4小时内组成释放，大约30-40%似乎被降解，其余的被纳入染色质囊泡，在尼古丁刺激下可以分泌CgA。新合成的DBH遵循类似的过程。新合成的CgA和DBH一旦进入染色质囊泡，在2-3天内表现为稳定，然后在3-4天的半衰期下降。牛肾上腺髓细胞原代培养是研究CgA和DBH合成调控因子及染色质囊泡形成的良好模型系统。
Primary cultures of bovine adrenal medullary cells synthesize chromogranin A (CgA) and dopamine beta-hydroxylase (DBH) and incorporate them into chromaffin vesicles. The incorporation of L-[35S]methionine into CgA, DBH, and total protein was approximately linear for 8 h at methionine concentrations of 12.5, 25, and 50 microM. Newly synthesized CgA and DBH were initially incorporated into vesicles of low buoyant density that matured over 24 h into vesicles having the greater buoyant density of chromaffin vesicles. Approximately 10% of the newly synthesized CgA is released constitutively within 4 h of formation, approximately 30-40% appears to be degraded, and the remainder is incorporated into chromaffin vesicles, which can secrete CgA in response to nicotinic stimulation. Newly synthesized DBH follows a similar course. Once incorporated into chromaffin vesicles, the newly synthesized CgA and DBH appear to be stable for 2-3 days and then decline with a half-time of 3-4 days. Primary cultures of bovine adrenal medullary cells are a good model system for studying factors regulating CgA and DBH synthesis and the formation of chromaffin vesicles.