THE SPECTROELECTROCHEMICAL DETERMINATION OF THE REDUCTION POTENTIAL OF DIFERRIC SERUM TRANSFERRIN
THE SPECTROELECTROCHEMICAL DETERMINATION OF THE REDUCTION POTENTIAL OF DIFERRIC SERUM TRANSFERRIN
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DOI：
10.1016/0167-4838(88)90301-9
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发表时间：
1988-08-31
期刊：
影响因子：
--
通讯作者：
RAYMOND, KN
中科院分区：
文献类型：
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作者：
KRETCHMAR, SA;REYES, ZE;RAYMOND, KN
使用甲基紫精介导电子转移到蛋白质，对铁转铁蛋白的形式还原电位进行了首次光谱电化学测量。这些计算考虑了亚铁转铁蛋白复合物的弱性质。在 pH 7.4、22℃ 的 0.100 M 三（羟甲基）氨基甲烷缓冲液中获得 -0.52(8) V 相对于标准氢电极的值。 C和2.0 M KCl。高离子强度对于实现还原是必要的，这支持了离子在转铁蛋白中铁的还原中发挥重要作用的观察结果。最后，描述了在分光光度池中的金电极处进行甲基紫精还原的程序。
The first spectroelectrochemical measurement of the formal reduction potential of iron transferrin has been carried out using methyl viologen to mediate electron transfer to the protein. These calculations take into consideration the weak nature of the ferrous transferrin complex. A value of -0.52(8) V vs. the normal hydrogen electrode was obtained in 0.100 M tris(hydroxymethyl)aminomethane buffer at pH 7.4, 22.degree. C, and 2.0 M KCl. A high ionic strength was necessary to effect reduction, supporting the observation that ions play an important role in the reduction of iron in transferrin. Finally, a procedure for carrying out the reduction of methyl viologen at a gold electrode in a spectrophotometric cell is described.