Biochip technologies in cancer research
Biochip technologies in cancer research
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DOI：
10.3109/07853890109002069
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发表时间：
2001-03-01
影响因子：
4.4
通讯作者：
Kallioniemi, OP
中科院分区：
文献类型：
--
作者：
Kallioniemi, OP
高通量“生物芯片”技术的发展大大提高了我们研究生物学和探索疾病分子基础的能力。生物芯片能够以小型化的形式进行大规模并行分子分析，具有非常高的通量。本文将重点介绍各种生物芯片技术在癌症研究中的应用，包括1）使用单核苷酸多态性（SNP）微阵列分析疾病易感性，2）使用cDNA微阵列分析全球基因表达模式，3）使用蛋白质组学生物芯片分析蛋白质的浓度、功能活性或相互作用，和4）通过使用组织微阵列，细胞类型或组织以及与分子靶相关的临床终点。可以预测个体癌症风险，在将来，通过关键基因中多个SNPs的微阵列图谱进行准确估计。通过生物芯片读出成千上万的基因和蛋白质的活性水平，将有助于癌症的诊断。生物芯片诊断与信息学解决方案相结合将成为癌症患者个性化治疗决策的基础。
Development of high-throughput 'biochip' technologies has dramatically enhanced our ability to study biology and explore the molecular basis of disease. Biochips enable massively parallel molecular analyses to be carried out in a miniaturized format with a very high throughput. This review will highlight applications of the various biochip technologies in cancer research, including analysis of 1) disease predisposition by using single-nucleotide polymorphism (SNP) microarrays, 2) global gene expression patterns by cDNA microarrays, 3) concentrations, functional activities or interactions of proteins with proteomic biochips, and 4) cell types or tissues as well as clinical endpoints associated with molecular targets by using tissue microarrays, One can predict that individual cancer risks can, in the future, be estimated accurately by a microarray profile of multiple SNPs in critical genes. Diagnostics of cancer will be facilitated by biochip readout of activity levels of thousands of genes and proteins. Biochip diagnostics coupled with informatics solutions will form the basis of individualized treatment decisions for cancer patients.