hsa-miR -7 Is a Potential Biomarker for Idiopathic Inflammatory Myopathies with Interstitial Lung Disease in Humans
hsa-miR -7 Is a Potential Biomarker for Idiopathic Inflammatory Myopathies with Interstitial Lung Disease in Humans
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hsa-miR -7 是人类特发性炎症性肌病伴间质性肺病的潜在生物标志物
DOI：
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发表时间：
2018
影响因子：
0.8
通讯作者：
Qi Fang
中科院分区：
文献类型：
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作者：
Liqiang Yu;Jie Li;Yujue Chen;Juean Jiang;Qi Fang
目标。microRNAs （miRNAs）已被确定为多种疾病的生物标志物。然而,。循环mirna在特发性炎症性肌病（IIM）诊断中的意义。仍然未知。在这项研究中，我们旨在探讨mirna作为潜在生物标志物的意义。用于预测IIM和间质性肺疾病（ILD）患者。方法。从43例IIM患者和43例健康人血浆中分离总RNA。分析mirna的表达。miRNA芯片，qRT-PCR验证。结果。微阵列显示更多的差异表达。与健康对照组相比，IIM患者循环mirna含量明显增加（P<0.05）。中存在的证实。IIM患者血浆样本中miR-7和miR-21水平发生显著变化。与健康对照组比较（P<0.05）。然而，在每个IIM中只有miR-7是唯一低表达的miRNA。IIM/ILD患者中表达量低于非ILD患者（P<0.05）。区分IIM/ILD与IIM无ILD的曲线下面积（AUC）为0.8978。95%置信区间为0.7961 ~ 0.9995。受试者工作特性（ROC）曲线。分析显示miR-7截断值为0.0063。此外，AUC分析显示了miR-7和miR-21。作为IIM患者健康对照的生物标志物具有诊断价值；而miR-7更为敏感。miR-7是一种潜在的生物标志物，可以作为IIM患者的诊断指标。区分IIM/ILD患者和IIM无ILD患者。
Objectives. microRNAs (miRNAs) have been identified as biomarkers for various diseases. However,.the significance of circulating miRNAs for the diagnosis of idiopathic inflammatory myopathies (IIM).is still unknown. In this study, we aim to investigate the significance of miRNAs as potential biomarkers.for predicting the patients with IIM and interstitial lung disease (ILD). Methods. Total RNA was isolated.from plasma of 43 IIM patients and 43 healthy people. The expression of miRNAs was analyzed by.miRNA microarray and validated by qRT-PCR. Results. microarray showed more differentially expressed.circulating miRNAs were found in IIM patients compared with healthy controls (P<0.05). qRT-PCR confirmed.miR-7 and miR-21 showed significantly changed levels in plasma samples between IIM patients.and healthy controls (P<0.05). However, only miR-7 was the sole miRNA lower expression in each IIM.patient (P<0.05), which is also lower expression in IIM/ILD patients than that without ILD (P<0.05)..And the area under curve (AUC) for distinguishing IIM/ILD patients from IIM without ILD is 0.8978,.and the 95% confidence interval is 0.7961 to 0.9995. The receiver operating characteristic (ROC) curve.analysis showed miR-7 cutoff value was 0.0063. Further, AUC analysis showed both miR-7 and miR-21.have diagnostic value as biomarkers for IIM patients form health controls; however, miR-7 is more sensitive..Conclusion. miR-7 is a potential biomarker as a diagnostic indicator for IIM patients and can be used.to distinguish IIM/ILD patients from IIM without ILD.