Phosphoproteomic analysis of longissimus lumborum of different altitude yaks.
Phosphoproteomic analysis of longissimus lumborum of different altitude yaks.
复制标题
DOI：
10.1016/j.meatsci.2019.108019
复制
发表时间：
2019-12
期刊：
影响因子：
7.1
通讯作者：
Yayuan Yang;Ling Han;Qunli Yu;Yongfang Gao;Rende Song;Suonan Zhao
中科院分区：
文献类型：
--
作者：
Yayuan Yang;Ling Han;Qunli Yu;Yongfang Gao;Rende Song;Suonan Zhao
高海拔地区牦牛对低氧环境有较好的适应性。然而，在低氧环境中调节肌肉蛋白质表达的机制还不完全清楚。为了探讨调节死后变化的机制，对两种不同海拔饲养的牦牛肌肉进行了定量磷酸蛋白质组学分析。结果表明，在高原牦牛中共鉴定出475个差异表达蛋白（DEPS），其中439个表达上调，36个表达下调。经生物信息学分析，筛选出26个与能量代谢和低氧适应相关的磷蛋白。此外，一些糖酵解酶被检测到差异磷酸化。两组蛋白磷酸化水平的差异可能是参与肌肉低氧适应调节的关键因素。目前的研究结果可以提供蛋白质组学的见解发生在牦牛肌肉在不同海拔高度的变化，并可能是一个有价值的资源，为未来的调查。
Yaks in high altitude regions display good adaptability to hypoxic environment. However, the mechanism involved in regulating muscle protein expression in hypoxic environment is not completely clear yet. To explore the mechanisms modulating postmortem alterations, quantitative phosphoproteomic analysis was performed on muscles of yaks raised at two different altitudes. The results indicated that 475 differentially expressed proteins (DEPS) were identified in high-altitude yaks, among which, 439 DEPs were up-regulated and 36 DEPs were down-regulated. Of these, 26 phosphoproteins clustered into energy metabolism and hypoxic adaption were selected after bioinformatics analysis. In addition, some glycolytic enzymes were detected to be differentially phosphorylated. The difference in protein phosphorylation levels between the two groups may be the key factor involved in the regulation of muscle hypoxic adaption. The present results could provide proteomic insights into changes occurring in yak muscles at different altitudes and may be a valuable resource for future investigations.