Comparative genomic analysis shows that Streptococcus suis meningitis isolate SC070731 contains a unique 105K genomic island.
Comparative genomic analysis shows that Streptococcus suis meningitis isolate SC070731 contains a unique 105K genomic island.
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DOI：
10.1016/j.gene.2013.11.044
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发表时间：
2014-02
期刊：
影响因子：
3.5
通讯作者：
Zongfu Wu;Weixue Wang;Min Tang;Jing Shao;C. Dai;Wei Zhang;H. Fan;Huochun Yao;Jie Zong
中科院分区：
文献类型：
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作者：
Zongfu Wu;Weixue Wang;Min Tang;Jing Shao;C. Dai;Wei Zhang;H. Fan;Huochun Yao;Jie Zong
猪链球菌（Streptococcus suis，SS）是一种重要的猪源性病原菌，偶尔会引起人类严重感染。SS感染可导致猪和人的脑膜炎。SS的致病机制知之甚少。在这里，我们提供了完整的基因组序列的S。从患脑膜炎的猪中分离的猪血清2型（SS 2）菌株SC 070731。染色体长度为2，138，568 bp。预测的蛋白质编码序列有1933个，96.7%（57/59）的已知毒力相关基因存在于基因组中。在斑马鱼感染模型中，SC 070731株的毒力与SS 2强毒株HA 9801和ZY 05719相似，但强于SS 2强毒株P1/7。比较基因组分析揭示了一个独特的105 K基因组岛的菌株SC 070731，是不存在的其他七个测序的SS 2菌株。对105 K基因组岛的进一步分析表明，它含有一个完整的nisin基因座，与S. uberisstrain 42中的nisin U基因座相似，是一个类似于S.噬菌体PH 10和几个抗生素抗性基因。105 K基因组岛中的几种蛋白质，包括乳链菌肽和RelBE毒素-抗毒素系统，有助于细菌在其他病原菌中的适应性和毒力。进一步研究新发现的基因产物，包括四个推定的新的毒力相关表面蛋白，将提高我们对SS发病机制的理解。
Streptococcus suis(SS) is an important swine pathogen worldwide that occasionally causes serious infections in humans. SS infection may result in meningitis in pigs and humans. The pathogenic mechanisms of SS are poorly understood. Here, we provide the complete genome sequence ofS. suisserotype 2 (SS2) strain SC070731 isolated from a pig with meningitis. The chromosome is 2,138,568 bp in length. There are 1933 predicted protein coding sequences and 96.7% (57/59) of the known virulence-associated genes are present in the genome. Strain SC070731 showed similar virulence with SS2 virulent strains HA9801 and ZY05719, but was more virulent than SS2 virulent strain P1/7 in the zebrafish infection model. Comparative genomic analysis revealed a unique 105 K genomic island in strain SC070731 that is absent in seven other sequenced SS2 strains. Further analysis of the 105 K genomic island indicated that it contained a complete nisin locus similar to the nisin U locus inS. uberisstrain 42, a prophage similar toS. oralisphage PH10 and several antibiotic resistance genes. Several proteins in the 105 K genomic island, including nisin and RelBE toxin–antitoxin system, contribute to the bacterial fitness and virulence in other pathogenic bacteria. Further investigation of newly identified gene products, including four putative new virulence-associated surface proteins, will improve our understanding of SS pathogenesis.