STIMULATION OF HUMAN LYMPHOCYTES-B BY ANTIBODIES TO IGM AND IGG - FUNCTIONAL EVIDENCE FOR THE EXPRESSION OF IGG ON LYMPHOCYTE-B SURFACE-MEMBRANES
STIMULATION OF HUMAN LYMPHOCYTES-B BY ANTIBODIES TO IGM AND IGG - FUNCTIONAL EVIDENCE FOR THE EXPRESSION OF IGG ON LYMPHOCYTE-B SURFACE-MEMBRANES
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DOI：
10.1016/0090-1229(80)90042-2
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
KUNKEL, HG
中科院分区：
文献类型：
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作者：
CHIORAZZI, N;FU, SM;KUNKEL, HG
分离的人扁桃体和外周血B淋巴细胞经F(ab‘)2片段亲和纯化的Ig M抗体、Ig G抗体、Fab抗体、Kappa抗体作用后，在体外被诱导增殖。还有.Lambda。链式行列式。在含有抗IgA抗体的培养物中观察到低水平的DNA合成，而在抗IGD刺激的培养物中没有检测到DNA合成。二价抗体是原始细胞发生所必需的。这些增殖反应是T[胸腺来源的]细胞不依赖的，并且容易受到过多单核细胞的抑制。它们在不含FCS[胎牛血清]或2-巯基乙醇的培养中是可以诱导的。高特异性抗体可诱导显著增殖，其程度与抗-IgM相似。进一步的分离研究表明，抗-Ig G反应细胞主要包含在表面的IgM+B细胞群中。所有抗体制剂均未诱导B细胞向抗体产生细胞分化。反.MU。抗体完全阻断了商陆丝裂原[PWM]刺激的未分离扁桃体单个核细胞的Ig合成。抗免疫球蛋白抗体类似地抑制脉宽调制诱导的抗体产生，尽管这在免疫球蛋白反应中最为明显。相反，抗IGD抗体既不能抑制Ig的产生，又在某些情况下导致Ig合成水平的增加。免疫球蛋白G分子明显与某些B细胞表面膜紧密结合，并与免疫球蛋白M共表达。这些观察进一步强调了表面免疫球蛋白M和免疫球蛋白G与表面免疫球蛋白D在B细胞增殖和分化中的不同功能。
Isolated human tonsillar and peripheral blood B [bone marrow-derived] lymphocytes were induced to proliferate in vitro after exposure to F(ab'')2 fragments of affinity purified antibodies to Ig[immunoglobulin]M, IgG, Fab, .kappa. and .lambda. chain determinants. Low levels of DNA synthesis were observed in cultures containing anti-IgA antibodies, whereas DNA synthesis was not detected in cultures stimulated with anti-IgD. Divalent antibodies were essential for the generation of blastogenesis. These proliferative responses were T [thymus-derived] cell independent and susceptible to suppression by excess numbers of monocytes. They were elicitable in cultures not containing FCS [fetal calf serum] or 2-mercaptoethanol. Highly specific antibodies to IgG induced marked proliferation; this was similar in degree to that induced by anti-IgM. Subfractionation studies demonstrated that the anti-IgG responsive cells were contained to a major extent within the surface IgM+ B cell population. None of the antibody preparations elicited B cell differentiation to antibody producing cells. Anti-.mu. antibodies completley abrogated Ig synthesis by pokeweed mitogen[PWM]-stimulated cultures of unseparated tonsillar mononuclear cells. Anti-IgG antibodies similarly suppressed PWM-induced antibody production, although this was most apparent on the IgG response. In contrast, anti-IgD antibodies both failed to suppress Ig production and in certain instances resulted in an increased level of Ig synthesis. IgG molecules are apparently intimately associated with the surface membrane of some B cells and coexpression of IgG with IgM occurs. The observations emphasize further the divergent functional roles which surface IgM and IgG vs. surface IgD have in B cell proliferation and differentiation.