A ratiometric fluorescent strategy based on copper nanoclusters/carbon dots for sensitive detection of doxorubicin
A ratiometric fluorescent strategy based on copper nanoclusters/carbon dots for sensitive detection of doxorubicin
复制标题
基于铜纳米簇/碳点的比率荧光策略用于灵敏检测阿霉素
DOI：
10.1002/bio.4230
复制
发表时间：
2022
期刊：
影响因子：
2.9
通讯作者：
Fubing Xiao
中科院分区：
文献类型：
--
作者：
Feifei Li;Pengfei Fan;Xinbei Chen;Xi Lin;Can Liu;Congcong Hu;Shengyuan Yang;Fubing Xiao
阿霉素(DOX)的灵敏检测是临床治疗的关键。通过设计比率荧光探针，建立了一种基于内滤波效应(IFE)的比率荧光策略用于DOX的灵敏检测。在DOX存在下，铜纳米团簇(CuNC)在485 nm处的荧光强度降低，而碳点在560 nm处的荧光强度增加。因此，可以通过测量560 nm和485 nm的荧光强度之比(F560/F485)来定量检测DOX。F560/F485比值与DOX浓度在1.0×10~(-8)M~1.0×10~(-4)M范围内呈线性关系，检出限为3.7 nm。此外，该方法还成功地应用于人体血浆样品中DOX的分析，为药物安全管理提供了有效的平台。
Sensitive detection of doxorubicin (DOX) is critical for clinical theranostics. A novel ratiometric fluorescence strategy based on the inner filter effect (IFE) has been established for the sensitive detection of DOX by designing a ratiometric fluorescence probe. In the presence of DOX, the fluorescence intensity of copper nanoclusters (CuNCs) at 485 nm decreases, and the fluorescence intensity of carbon dots at 560 nm increases. Therefore, DOX can be quantitatively detected by measuring the ratio of the fluorescence intensities at 560 and 485 nm (F560 /F485 ). The F560 /F485 ratio exhibits a linear correlation with the DOX concentration in the range from 1.0 × 10-8 M to 1.0 × 10-4 M with the detection limit of 3.7 nM. Furthermore, this method was also successfully applied to the analysis of DOX in human plasma samples, affording an effective platform for drug safety management.