THE DEFINITION OF GENERALIZED HELICOIDAL PARAMETERS AND OF AXIS CURVATURE FOR IRREGULAR NUCLEIC-ACIDS
THE DEFINITION OF GENERALIZED HELICOIDAL PARAMETERS AND OF AXIS CURVATURE FOR IRREGULAR NUCLEIC-ACIDS
复制标题
DOI：
10.1080/07391102.1988.10506483
复制
发表时间：
1988-08-01
影响因子：
4.4
通讯作者：
SKLENAR, H
中科院分区：
文献类型：
--
作者：
LAVERY, R;SKLENAR, H
提出了一种算法，它解决了获得一个严格的螺旋描述的不规则的核酸片段的问题。这种方法的核心是定义一个函数，该函数同时描述所讨论的核酸片段的曲率和螺旋参数沿该片段沿着的相应逐步变化。该函数的最小化导致这两个组分之间的片段的构象不规则性的最佳分布。此外，它表明，这种方法可以同样容易地应用于单链或双链核酸。该分析的结果产生单个碱基/碱基对的绝对螺旋参数和连续碱基/碱基对之间的相对螺旋参数以及螺旋轴的总体轨迹。这种数学方法的可能性证明了与计算机程序的帮助下，被称为“曲线”，这是适用于研究的一些不同的核酸结构。
An algorithm is presented which solves the problem of obtaining a rigorous helicoidal description of an irregular nucleic acid segment. Central to this approach is the definition of a function describing simultaneously the curvature of the nucleic acid segment in question and the corresponding stepwise variation of helicoidal parameters along the segment. Minimisation of this function leads to an optimal distribution of the conformational irregularity of the segment between these two components. Further, it is shown that this approach can be applied equally easily to single or double stranded nucleic acids. The results of this analysis yield both the absolute helicoidal parameters of individual bases/base pairs and the relative helicoidal parameters between successive bases/base pairs as well as the overall locus of the helical axis. The possibilities of this mathematical approach are demonstrated with the help of a computer program termed "Curves" which is applied to the study of a number of different nucleic acid structures.