DEVELOPMENTAL-CHANGES IN THE OVARIAN FOLLICULAR BASAL LAMINA DETECTED BY IMMUNOFLUORESCENCE AND ELECTRON-MICROSCOPY
DEVELOPMENTAL-CHANGES IN THE OVARIAN FOLLICULAR BASAL LAMINA DETECTED BY IMMUNOFLUORESCENCE AND ELECTRON-MICROSCOPY
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DOI：
10.1177/31.5.6341456
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发表时间：
1983-01-01
影响因子：
3.2
通讯作者：
WICHA, M
中科院分区：
文献类型：
--
作者：
BAGAVANDOSS, P;MIDGLEY, AR;WICHA, M
使用抗IV型胶原、层粘连蛋白和纤连蛋白的抗体，通过免疫荧光显微镜和EM研究了[大鼠]发育卵泡基底层（BL）的变化。通过顺序给予雌二醇、FSH和人绒毛膜促性腺激素（hCG）诱导未成熟大鼠的卵巢发育。在用雌二醇和FSH处理的健康卵泡中观察到连续BL。免疫荧光法测定，层粘连蛋白，IV型胶原蛋白，纤连蛋白仅限于BL和卵泡膜，但不颗粒。当卵泡被允许进行闭锁或诱导排卵与hCG，BL变得支离破碎。健康卵泡、闭锁卵泡和黄体化卵泡的电子显微镜检查证实了这一点，结果表明，健康卵泡的BL是连续的，而闭锁卵泡和黄体化卵泡的BL是碎片化的。闭锁也与卵泡膜细胞进入卵泡有关。在闭锁和排卵过程中，健康卵泡中完整的BL发生了广泛的变化。
Alterations in the basal lamina (BL) of [rat] developing follicles were studied by immunofluorescent microscopy using antibodies against type IV collagen, laminin and fibronectin, and by EM. Ovarian development was induced in immature rats by sequential administration of estradiol, FSH and human chorionic gonadotropin (hCG). A continuous BL was observed in healthy follicles treated with estradiol and FSH. As determined by immunofluorescence, laminin, type IV collagen, and fibronectin were restricted to the BL and the theca but not to the granulosa. When follicles were allowed to undergo atresia or induced to ovulate with hCG, the BL became fragmented. This was confirmed by electron microscopy of healthy, atretic, and luteinizing follicles which showed that in healthy follicles the BL was continuous, whereas in both atretic and luteinizing follicles, it was fragmented. Atresia was also associated with the penetration of thecal cells into the follicles. The intact BL present in healthy follicles undergo extensive changes during atresia and ovulation.