The forkhead protein Foxj1 specifies node-like cilia in Xenopus and zebrafish embryos.
The forkhead protein Foxj1 specifies node-like cilia in Xenopus and zebrafish embryos.
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DOI：
10.1038/ng.267
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发表时间：
2008-12
期刊：
影响因子：
30.8
通讯作者：
Kintner C
中科院分区：
文献类型：
--
作者：
Stubbs JL;Oishi I;Izpisúa Belmonte JC;Kintner C
产生向左流体流动的纤毛细胞已被认为可以介导许多脊椎动物胚胎的左右模式。这些细胞上的纤毛结合了初级感觉纤毛和运动纤毛的特征，但如何指定这种纤毛亚型尚不清楚。我们通过分析 FoxJ1 的爪蟾和斑马鱼同源物来解决这个问题，FoxJ1 是小鼠多纤毛细胞中纤毛发生所必需的叉头转录因子。我们发现，非洲爪蟾胃腔顶板 (GRP) 和斑马鱼 Kupffer 囊泡 (KV) 上左右模式下的纤毛在 FoxJ1 变形体中严重缩短或无法形成。我们还表明，错误表达 XFoxJ1 足以诱导青蛙胚胎中异位 GRP 样纤毛的形成。微阵列分析表明，XFoxJ1 通过上调运动纤毛基因的表达来诱导纤毛的形成。这些结果表明 FoxJ1 是指定左右图案中使用的纤毛的关键决定因素。
Ciliated cells that produce a leftward fluid flow have been proposed to mediate left-right patterning in many vertebrate embryos. The cilia on these cells combine features of primary sensory and motile cilia, but how this cilia subtype is specified is unknown. We address this issue by analyzing the Xenopus and Zebrafish homologs of FoxJ1, a forkhead transcription factor necessary for ciliogenesis in multi-ciliate cells of the mouse. We show that the cilia that underlie left-right patterning on the Xenopus gastrocoel roof plate (GRP) and Zebrafish Kupffer's vesicle (KV) are severely shortened or fail to form in FoxJ1 morphants. We also show that misexpressing XFoxJ1 is sufficient to induce ectopic GRP-like cilia formation in frog embryos. Microarray analysis indicates that XFoxJ1 induces the formation of cilia by upregulating the expression of motile cilia genes. These results indicate that FoxJ1 is a critical determinant in specifying cilia used in left-right patterning.