ENZYME SENSOR FOR L-LACTATE USING DIFFERENTIAL-PULSE AMPEROMETRIC DETECTION
ENZYME SENSOR FOR L-LACTATE USING DIFFERENTIAL-PULSE AMPEROMETRIC DETECTION
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DOI：
10.1007/bf00321644
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发表时间：
1995-01-01
期刊：
影响因子：
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通讯作者：
HASEBE, K
中科院分区：
文献类型：
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作者：
HIKIMA, S;KAKIZAKI, T;HASEBE, K
利用差分脉冲（DP）安培检测技术，研制了一种酶传感器，用于测量酶反应消耗的氧化型β -烟酰胺腺嘌呤二核苷酸（NAD(+)）的还原电流。这种生物传感器在防止诸如抗坏血酸和尿酸等电氧化物质的干扰方面具有明显的优势，与传统的直流安培检测器相比，具有更高的灵敏度和选择性。该传感器的线性检测范围为5.0 × 10(-5) ~ 5.0 × 10(-4) mol/l，在2.5 × 10(-4) mol/l时的相对标准偏差（rsd）为5.0%。
An enzyme sensor using differential pulse (DP) amperometric detection has been developed based on the measurement of the reduction current of the oxidized form of beta-nicotinamide adenine dinucleotide (NAD(+)) consumed by an enzyme reaction. This biosensor has the definite advantage to prevent interference caused by electrooxidative species such as ascorbate and uric acid and exhibits higher sensitivity and selectivity in comparison to the classical DC amperometric detector. The linear detection range of this biosensor was 5.0 x 10(-5)-5.0 x 10(-4) mol/l and the relative standard deviation (R.S.D.) at 2.5 x 10(-4) mol/l was 5.0%.