Reduced Diversity and Imbalance of Fecal Microbiota in Patients with Ulcerative Colitis
Reduced Diversity and Imbalance of Fecal Microbiota in Patients with Ulcerative Colitis
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DOI：
10.1007/s10620-012-2236-y
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发表时间：
2012-11-01
影响因子：
3.1
通讯作者：
Yasutomo, Koji
中科院分区：
文献类型：
--
作者：
Nemoto, Hideyuki;Kataoka, Keiko;Yasutomo, Koji
临床观察和实验性结肠炎模型已经表明肠道细菌在溃疡性结肠炎（UC）病因学中的重要性，但致病细菌尚未被确定。为了确定肠道细菌与UC的关系，对UC患者和健康成人的粪便微生物群和其他成分进行了比较。通过使用末端限制性片段长度多态性（T-RFLP）、实时PCR和培养来分析粪便微生物群。测定了反映肠道环境指标的有机酸、吲哚、氨、pH值和水分的浓度，并与健康对照组数据进行比较。T-RFLP数据将UC患者分为4个聚类，其中1个聚类为健康受试者。UC患者粪便微生物群的多样性显著降低。UC患者的拟杆菌和梭菌亚群XIVab明显少于健康受试者，肠球菌的数量高于健康受试者。缓解期UC患者粪便中有机酸浓度显著降低。UC患者肠道环境失衡，粪便微生物群多样性减少，主要厌氧菌（拟杆菌属和梭菌属亚群XIVab）水平较低，有机酸浓度较低。
Clinical observations and experimental colitis models have indicated the importance of intestinal bacteria in the etiology of ulcerative colitis (UC), but a causative bacterial agent has not been identified.To determine how intestinal bacteria are associated with UC, fecal microbiota and other components were compared for UC patients and healthy adults.Fresh feces were collected from 48 UC patients. Fecal microbiota were analyzed by use of terminal-restriction fragment length polymorphism (T-RFLP), real-time PCR, and culture. The concentrations of organic acids, indole, and ammonia, and pH and moisture, which are indicators of the intestinal environment, were measured and compared with healthy control data.T-RFLP data divided the UC patients into four clusters; one cluster was obtained for healthy subjects. The diversity of fecal microbiota was significantly lower in UC patients. There were significantly fewer Bacteroides and Clostridium subcluster XIVab, and the amount of Enterococcus was higher in UC patients than in healthy subjects. The fecal concentration of organic acids was significantly lower in UC patients who were in remission.UC patients have imbalances in the intestinal environment-less diversity of fecal microbiota, lower levels of major anaerobic bacteria (Bacteroides and Clostridium subcluster XIVab), and a lower concentration of organic acids.